Kleessen Brigitta, Blaut Michael
Veterinary Faculty, Institute of Bacteriology and Mycology, University of Leipzig, Germany.
Br J Nutr. 2005 Apr;93 Suppl 1:S35-40. doi: 10.1079/bjn20041346.
Non-digestible inulin-type fructans, such as oligofructose and high-molecular-weight inulin, have been shown to have the ability to alter the intestinal microbiota composition in such a way that members of the microbial community, generally considered as health-promoting, are stimulated. Bifidobacteria and lactobacilli are the most frequently targeted organisms. Less information exists on effects of inulin-type fructans on the composition, metabolism and health-related significance of bacteria at or near the mucosa surface or in the mucus layer forming mucosa-associated biofilms. Using rats inoculated with a human faecal flora as an experimental model we have found that inulin-type fructans in the diet modulated the gut microbiota by stimulation of mucosa-associated bifidobacteria as well as by partial reduction of pathogenic Salmonella enterica subsp. enterica serovar Typhimurium and thereby benefit health. In addition to changes in mucosal biofilms, inulin-type fructans also induced changes in the colonic mucosa stimulating proliferation in the crypts, increasing the release of mucins, and altering the profile of mucin components in the goblet cells and epithelial mucus layer. These results indicate that inulin-type fructans may stabilise the gut mucosal barrier. Dietary supplementation with these prebiotics could offer a new approach to supporting the barrier function of the mucosa.
不可消化的菊粉型果聚糖，如低聚果糖和高分子量菊粉，已被证明有能力改变肠道微生物群的组成，从而刺激通常被认为具有健康促进作用的微生物群落成员。双歧杆菌和乳酸菌是最常被靶向的微生物。关于菊粉型果聚糖对黏膜表面或形成黏膜相关生物膜的黏液层中或附近细菌的组成、代谢及健康相关意义的影响，现有信息较少。以接种了人类粪便菌群的大鼠作为实验模型，我们发现饮食中的菊粉型果聚糖通过刺激黏膜相关双歧杆菌以及部分减少致病性肠炎沙门氏菌肠炎亚种鼠伤寒血清型来调节肠道微生物群，从而有益于健康。除了黏膜生物膜的变化外，菊粉型果聚糖还诱导结肠黏膜发生变化，刺激隐窝中的细胞增殖，增加黏蛋白的释放，并改变杯状细胞和上皮黏液层中黏蛋白成分的分布。这些结果表明菊粉型果聚糖可能稳定肠道黏膜屏障。膳食补充这些益生元可能为支持黏膜的屏障功能提供一种新方法。
