Ariaee Amin, Wardill Hannah R, Wignall Anthony, Prestidge Clive A, Joyce Paul
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, SA 5000, Australia.
School of Biomedicine, The University of Adelaide, Adelaide, SA 5000, Australia.
Foods. 2024 Mar 28;13(7):1039. doi: 10.3390/foods13071039.
Inulin, a non-digestible polysaccharide, has gained attention for its prebiotic properties, particularly in the context of obesity, a condition increasingly understood as a systemic inflammatory state linked to gut microbiota composition. This study investigates the short-term protective effects of inulin with different degrees of polymerization (DPn) against metabolic health deterioration and gut microbiota alterations induced by a high-fat diet (HFD) in Sprague Dawley rats. Inulin treatments with an average DPn of 7, 14, and 27 were administered at 1 g/kg of bodyweight to HFD-fed rats over 21 days. Body weight, systemic glucose levels, and proinflammatory markers were measured to assess metabolic health. Gut microbiota composition was analyzed through 16S rRNA gene sequencing. The results showed that inulin significantly reduced total weight gain and systemic glucose levels, suggesting a DPn-specific effect on metabolic health. The study also observed shifts in gut microbial populations, with inulin promoting several beneficial taxa from the genera, whilst inducing a unique microbial composition compared to medium-chain (DPn 14) and long-chain inulin (DPn: 27). However, the impact of inulin on proinflammatory markers and lipid metabolism parameters was not statistically significant, possibly due to the short study duration. Inulin with a higher DPn has a more pronounced effect on mitigating HFD-induced metabolic health deterioration, whilst inulin is particularly effective at inducing healthy microbial shifts. These findings highlight the benefits of inulin as a dietary adjuvant in obesity management and the importance of DPn in optimizing performance.
菊粉是一种不可消化的多糖，因其益生元特性而受到关注，尤其是在肥胖背景下，肥胖越来越被认为是一种与肠道微生物群组成相关的全身性炎症状态。本研究调查了不同聚合度（DPn）的菊粉对高脂饮食（HFD）诱导的Sprague Dawley大鼠代谢健康恶化和肠道微生物群改变的短期保护作用。将平均DPn为7、14和27的菊粉以1 g/kg体重的剂量给予HFD喂养的大鼠，持续21天。测量体重、全身血糖水平和促炎标志物以评估代谢健康。通过16S rRNA基因测序分析肠道微生物群组成。结果表明，菊粉显著降低了总体重增加和全身血糖水平，表明对代谢健康有DPn特异性影响。该研究还观察到肠道微生物种群的变化，菊粉促进了几个属的有益类群，同时与中链（DPn 14）和长链菊粉（DPn：27）相比诱导了独特的微生物组成。然而，菊粉对促炎标志物和脂质代谢参数的影响没有统计学意义，可能是由于研究持续时间较短。较高DPn的菊粉对减轻HFD诱导的代谢健康恶化有更显著的作用，而菊粉在诱导健康的微生物变化方面特别有效。这些发现突出了菊粉作为肥胖管理膳食佐剂的益处以及DPn在优化性能方面的重要性。
