Stolz Andreas, Bürger Sibylle, Kuhm Andrea, Kämpfer Peter, Busse Hans-Jürgen
Institut für Mikrobiologie, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32 (IFZ), D-35392 Giessen, Germany.
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1077-1081. doi: 10.1099/ijs.0.63466-0.
The taxonomic position of a Pseudomonas-like strain, designated BN9(T), was investigated. This strain had previously been isolated as a 5-aminosalicylate-degrading organism from a 6-aminonaphthalene-2-sulphonate-degrading mixed bacterial culture. Previously, detection of ubiquinone Q-8, a polyamine pattern with putrescine, spermidine and 2-hydroxyputrescine as the major polyamines, and partial 16S rRNA gene sequencing had suggested that strain BN9(T) belongs to the 'Betaproteobacteria'. This was supported by sequencing the 16S rRNA gene, which demonstrated 94-96 % sequence similarity to different species of the genera Achromobacter, Alcaligenes and Bordetella, and suggested that strain BN9(T) represents a member of the family Alcaligenaceae. Different methods for the construction of phylogenetic dendrograms placed the strain separately from all other genera within the Alcaligenaceae. Fatty acid analysis demonstrated the presence of high concentrations of C(19 : 0) cyclo omega8c. On the basis of low 16S rRNA gene sequence similarity to other members of the Alcaligenaceae, fatty acid and polar lipid profiles, and other unique phenotypic properties of strain BN9(T), the creation of a new genus and species with the name Pusillimonas noertemannii gen. nov., sp. nov. is proposed. The type strain is BN9(T) (= DSM 10065(T) = NCIMB 14020(T)).
对一株命名为BN9(T)的类假单胞菌菌株的分类地位进行了研究。该菌株先前是从一种降解6-氨基萘-2-磺酸盐的混合细菌培养物中作为降解5-氨基水杨酸的微生物分离得到的。此前，对泛醌Q-8的检测、以腐胺、亚精胺和2-羟基腐胺为主要多胺的多胺模式以及16S rRNA基因部分测序表明，菌株BN9(T)属于“β-变形菌纲”。16S rRNA基因测序支持了这一结论，该测序表明其与无色杆菌属、产碱菌属和博德特氏菌属的不同种有94-96%的序列相似性，表明菌株BN9(T)代表产碱菌科的一个成员。构建系统发育树状图的不同方法将该菌株与产碱菌科内的所有其他属分开。脂肪酸分析表明存在高浓度的C(19 : 0)环ω8c。基于与产碱菌科其他成员较低的16S rRNA基因序列相似性、脂肪酸和极性脂质谱以及菌株BN9(T)的其他独特表型特征，提议创建一个新的属和种，命名为诺氏微小单胞菌（Pusillimonas noertemannii），新属，新种。模式菌株为BN9(T)（= DSM 10065(T) = NCIMB 14020(T)）。
