Yao Li, Jia Yan, Lai Yu-Han, Xue Fei, Wang Jia-Lian
College of Marine and Bio-Engineering, Yancheng Teachers University, Yancheng, Jiangsu 224000, PR China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu 210000, PR China.
Int J Syst Evol Microbiol. 2022 Apr;72(4). doi: 10.1099/ijsem.0.005323.
A novel pale white-pigmented bacterial strain designated YC-7-48 was isolated from activated sludge in China. Cells of the strain, which grew at 15-37 °C (optimum at 30 °C) and pH 6.0-9.0 (optimum at 7.0), were Gram-stain-negative, rod-shaped and motile. Strain YC-7-48 had 97.4-97.1% 16S rRNA gene sequence similarity to type strains of eight species in the genera , , , and of the family . Phylogenetic analysis based on 16S rRNA gene sequencing placed the strain on a separate branch in the genus and showed that it exhibited 97.4, 97.3 and 96.6% similarity to EBR-8-1, BN9 and 17-4A, respectively. The genome size of strain YC-7-48 was 3202438 bp, with 54.3 mol% G+C content. According to the genome analysis, YC-7-48 encodes several heavy metal resistance proteins and enzymes related to the metabolism of nicotine and aromatic compounds. The results of digital DNA-DNA hybridization and average nucleotide identity analyses based on whole genome sequences between strain YC-7-48 and the closely related strains indicated that the strain represented a new species of the genus . The chemotaxonomic results identified Q-8 as the predominant respiratory quinone, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and two unidentified aminolipids as the major polar lipids, and C (27.4 %), C cyclo (22.0 %), C (11.7 %) and C cyclo 8 (9.5 %) as the major fatty acids. Thus, based on morphological, chemotaxonomic and phylogenetic characterization and genomic data, we proposed that the isolate is a representative of a novel species named sp. nov., with the type strain YC-7-48 (=CGMCC 1.17466=KACC 21349).
从中国活性污泥中分离出一株新型的浅白色色素细菌菌株，命名为YC-7-48。该菌株细胞在15-37°C（最适温度30°C）和pH 6.0-9.0（最适pH 7.0）条件下生长，革兰氏染色阴性，杆状且具运动性。菌株YC-7-48与科、属中八个种的模式菌株的16S rRNA基因序列相似性为97.4-97.1%。基于16S rRNA基因测序的系统发育分析表明，该菌株在属内位于一个单独的分支上，并且分别显示出与EBR-8-1、BN9和17-4A的相似性为97.4%、97.3%和96.6%。菌株YC-7-48的基因组大小为3202438 bp，G+C含量为54.3 mol%。根据基因组分析，YC-7-48编码几种重金属抗性蛋白以及与尼古丁和芳香化合物代谢相关的酶。基于全基因组序列的数字DNA-DNA杂交和平均核苷酸同一性分析结果表明，该菌株代表了属内的一个新物种。化学分类学结果确定Q-8为主要呼吸醌，磷脂酰乙醇胺、磷脂酰单甲基乙醇胺、二磷脂酰甘油和两种未鉴定的氨基脂质为主要极性脂质，C（27.4%）、C环（22.0%）、C（11.7%）和C环8（9.5%）为主要脂肪酸。因此，基于形态学、化学分类学和系统发育特征以及基因组数据，我们提议该分离株是一个名为sp. nov.新物种的代表，模式菌株为YC-7-48（=CGMCC 1.17466=KACC 21349）。
