sp. nov., a novel member of the genus isolated from activated sludge.

A novel pale white-pigmented bacterial strain designated YC-7-48 was isolated from activated sludge in China. Cells of the strain, which grew at 15-37 °C (optimum at 30 °C) and pH 6.0-9.0 (optimum at 7.0), were Gram-stain-negative, rod-shaped and motile. Strain YC-7-48 had 97.4-97.1% 16S rRNA gene sequence similarity to type strains of eight species in the genera , , , and of the family . Phylogenetic analysis based on 16S rRNA gene sequencing placed the strain on a separate branch in the genus and showed that it exhibited 97.4, 97.3 and 96.6% similarity to EBR-8-1, BN9 and 17-4A, respectively. The genome size of strain YC-7-48 was 3202438 bp, with 54.3 mol% G+C content. According to the genome analysis, YC-7-48 encodes several heavy metal resistance proteins and enzymes related to the metabolism of nicotine and aromatic compounds. The results of digital DNA-DNA hybridization and average nucleotide identity analyses based on whole genome sequences between strain YC-7-48 and the closely related strains indicated that the strain represented a new species of the genus . The chemotaxonomic results identified Q-8 as the predominant respiratory quinone, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and two unidentified aminolipids as the major polar lipids, and C (27.4 %), C cyclo (22.0 %), C (11.7 %) and C cyclo 8 (9.5 %) as the major fatty acids. Thus, based on morphological, chemotaxonomic and phylogenetic characterization and genomic data, we proposed that the isolate is a representative of a novel species named sp. nov., with the type strain YC-7-48 (=CGMCC 1.17466=KACC 21349).