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耐碱纳氏菌属,新属,新种,从中国分离出的“草酸杆菌科”新成员。

Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China.

作者信息

Xu Ping, Li Wen-Jun, Tang Shu-Kun, Zhang Yu-Qin, Chen Guo-Zhong, Chen Hua-Hong, Xu Li-Hua, Jiang Cheng-Lin

机构信息

New Drug R & D, North China Pharmaceutic Corp., Shijiazhuang, 050015, People's Republic of China.

The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China.

出版信息

Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1149-1153. doi: 10.1099/ijs.0.63407-0.

Abstract

A taxonomic study was performed on strain YIM 31775(T), which was isolated from a soil sample collected from Yunnan Province, China. The isolate was chemo-organotrophic, aerobic and Gram-negative. Cells were short rods and motile, with one or more polar flagella. Growth temperature and pH ranged from 4 to 55 degrees C and 6.5 to 12.0, respectively; the optimum growth temperature and pH were 28-37 degrees C and 7.0-9.0, respectively. Q-8 was the predominant respiratory lipoquinone. The major fatty acids were C(16 : 1)omega7c (42.4 %) and C(16 : 0) (28.1 %). The DNA G + C content was 62.4 +/ -0.3 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM 31775(T) should be placed within the family 'Oxalobacteraceae', in which it formed a distinct lineage. Based on the high 16S rRNA gene sequence divergence and phenotypic characteristics, it is proposed that strain YIM 31775(T) should be classified as representing a novel member of the family 'Oxalobacteraceae', for which the name Naxibacter alkalitolerans gen. nov., sp. nov. is proposed. The type strain is YIM 31775(T) (= CCTCC AA 204003(T) = KCTC 12194(T)).

摘要

对菌株YIM 31775(T)进行了分类学研究,该菌株从中国云南省采集的土壤样本中分离得到。该分离株为化能有机营养型、好氧且革兰氏阴性。细胞为短杆状且具运动性,有一根或多根极生鞭毛。生长温度范围为4至55℃,pH范围为6.5至12.0;最适生长温度和pH分别为28 - 37℃和7.0 - 9.0。泛醌Q - 8是主要的呼吸脂醌。主要脂肪酸为C(16 : 1)ω7c(42.4%)和C(16 : 0)(28.1%)。DNA的G + C含量为62.4 ± 0.3 mol%。基于16S rRNA基因序列的系统发育分析表明,菌株YIM 31775(T)应归入“草酸杆菌科”,在该科中形成一个独特的分支。基于高16S rRNA基因序列差异和表型特征,建议将菌株YIM 31775(T)分类为代表“草酸杆菌科”的一个新成员,为此提出新属名Naxibacter alkalitolerans gen. nov.,新种名sp. nov.。模式菌株为YIM 31775(T)(= CCTCC AA 204003(T) = KCTC 12194(T))。

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