Thompson Ryan Michael, Fox Edward M, Koutsidis Georgios, Del Carmen Montero-Calasanz Maria
School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
Department of Applied Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK.
Int J Syst Evol Microbiol. 2025 Jun;75(6). doi: 10.1099/ijsem.0.006810.
The root nodules of remain a relatively understudied niche, with poorly described associated microbial communities. In this study, the isolate RTGN1 was recovered from root nodules collected from Gateshead, UK, and was identified as belonging to based on 16S rRNA gene similarity and phylogenomic placement. This isolate was polyphasically characterized, displaying the ability to grow between 12 and 28 °C and pH 6 and 8 and exhibiting the genes necessary to produce the polar lipids phosphatidylethanolamine and phosphatidylglycerophosphate, alongside DL-type peptidoglycan, which are diagnostic of . Overall genomic relatedness index values were below the cut-off value for delineating a novel species. As such, it is proposed that RTGN1 be recognized as the type strain (=CECT 30870=CABI 507287) of sp. nov. The RTGN1 isolate was screened using and methods and was found to possess a number of genes and pathways related to secondary metabolite production and plant growth promotion. Such genes may serve as an avenue of future study regarding biotechnological potential and use as a bioinoculant to increase phytoremediation efficiency.
[植物名称]的根瘤仍是一个研究相对较少的生态位，其相关微生物群落的描述也很匮乏。在本研究中，分离株RTGN1是从英国盖茨黑德采集的根瘤中获得的，基于16S rRNA基因相似性和系统基因组定位，它被鉴定为属于[某一属]。该分离株进行了多相表征，显示其能够在12至28°C以及pH值为6至8的条件下生长，并具有产生磷脂酰乙醇胺和磷脂酰甘油磷酸这两种极性脂质以及DL型肽聚糖所需的基因，这些是[某一属]的诊断特征。总体基因组相关性指数值低于划定一个新物种的临界值。因此，建议将RTGN1认定为[某一新物种]的模式菌株（=CECT 30870=CABI 507287）。使用[具体方法1]和[具体方法2]对RTGN1分离株进行筛选，发现它拥有许多与次生代谢产物产生和促进植物生长相关的基因和途径。这些基因可能成为未来关于生物技术潜力研究以及用作生物接种剂以提高植物修复效率的一个研究方向。
