Chen Guo-Hua, Xiong Lian-Shuang, Yang Rui, Zuo Shu-Ya, Yuan Qing, Wang Li-Song, Jiang Cheng-Lin, Wang Xin-Yu, Jiang Yi
The Key Lab for Research & Development of Actinomycete Resources, Yunnan Institute of Microbiology, Chenggong Campus of Yunnan University, Chenggong District, Kunming, 650500, Yunnan, China.
Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China.
Curr Microbiol. 2025 Aug 26;82(10):478. doi: 10.1007/s00284-025-04453-1.
A Gram-stain-positive bacterium, designated as YIM 134068, was isolated from a lichen. Strain YIM 134068 was non-motile, non-spore forming, coccus shaped, oxidase, and catalase positive. Cells could grow at 10-40 °C (optimum 28 °C) and pH 7.0-8.0 (optimum pH 7.0), and they could proliferate in the salinity range of 0-3.0% (w/v) NaCl. Strain YIM 134068 possessed MK-8 as the major respiratory quinone, and its predominant cellular fatty acids (> 10%) were summed feature 3 (Cω7c and/or Cω6c), CN alcohol, and C. Polar lipids comprised of phosphatidyl ethanolamine, two unidentified phospholipids, and seven unindentified phosphoglycolipids. Phylogenetic analysis using 16S rRNA gene sequence indicated that strain YIM 134068 shared the highest sequence similarities with Deinococcus aetherius ST0316 (93.2%) and Deinococcus aestuarii SYSU M49105 (92.1%). The DNA G + C content of YIM 134068 was 69.1% and the average nucleotide identity and digital DNA-DNA hybridization values between strain YIM 134068 and the reference strains were 82.6-83.0% and 26.3-26.7%, respectively. Based on the phylogenetic and phenotypic analyses, strain YIM 134068 is considered to represent a novel species within the genus Deinococcus, for which the name Deinococcus lichenicola sp. nov. is proposed. The type strain is YIM 134068 = CGMCC 1.61729 = MCCC 1K08693 = NBRC 117087.
从地衣中分离出一株革兰氏阳性菌，命名为YIM 134068。YIM 134068菌株无运动性，不形成芽孢，呈球菌状，氧化酶和过氧化氢酶呈阳性。细胞能在10-40℃（最适温度28℃）和pH 7.0-8.0（最适pH 7.0）下生长，并且能在0-3.0%（w/v）NaCl的盐度范围内增殖。YIM 134068菌株以MK-8作为主要呼吸醌，其主要细胞脂肪酸（>10%）为总和特征3（Cω7c和/或Cω6c）、CN醇和C。极性脂质由磷脂酰乙醇胺、两种未鉴定的磷脂和七种未鉴定的磷酸糖脂组成。使用16S rRNA基因序列进行的系统发育分析表明，YIM 134068菌株与嗜乙醚嗜皮菌ST0316（93.2%）和河口嗜皮菌SYSU M49105（92.1%）具有最高的序列相似性。YIM 134068的DNA G+C含量为69.1%，YIM 134068菌株与参考菌株之间的平均核苷酸同一性和数字DNA-DNA杂交值分别为82.6-83.0%和26.3-26.7%。基于系统发育和表型分析，YIM 134068菌株被认为代表嗜皮菌属内的一个新物种，为此提出新名称嗜皮菌地衣icola sp. nov.。模式菌株为YIM 134068 = CGMCC 1.61729 = MCCC 1K08693 = NBRC 117087。
