Vanparys Bram, Heylen Kim, Lebbe Liesbeth, De Vos Paul
Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1315-1318. doi: 10.1099/ijs.0.63635-0.
A Gram-negative, rod-shaped, non-spore-forming bacterium was isolated from a nitrifying inoculum. On the basis of 16S rRNA gene sequence similarity, LMG 22862(T) was shown to belong to the family Sphingobacteriaceae, related to Pedobacter africanus (98.0 %) and Pedobacter heparinus (97.6 %). The results of 16S rRNA gene sequence analysis, DNA-DNA hybridization, SDS-PAGE, analysis of the fatty acid composition and physiological and biochemical tests allowed genotypic and phenotypic differentiation of LMG 22862(T) from Pedobacter species with validly published names. LMG 22862(T) therefore represents a novel species within this genus, for which the name Pedobacter caeni sp. nov. is proposed, with the type strain LMG 22862(T) (=DSM 16990(T)).
从硝化接种物中分离出一种革兰氏阴性、杆状、不形成芽孢的细菌。基于16S rRNA基因序列相似性，LMG 22862(T)被证明属于鞘氨醇杆菌科，与非洲 Pedobacter africanus（98.0%）和肝素 Pedobacter heparinus（97.6%）相关。16S rRNA基因序列分析、DNA-DNA杂交、SDS-PAGE、脂肪酸组成分析以及生理生化测试结果使得LMG 22862(T)能够与已有效发表名称的 Pedobacter 属物种在基因型和表型上进行区分。因此，LMG 22862(T)代表了该属内的一个新物种，为此提出名称 Pedobacter caeni sp. nov.，模式菌株为LMG 22862(T)（=DSM 16990(T)）。
