Garcia Valerie, Furuya Kanji, Carr Antony M
Genome Damage and Stability Center, University of Sussex, Brighton, Sussex BN1 9RQ, UK.
DNA Repair (Amst). 2005 Nov 21;4(11):1227-39. doi: 10.1016/j.dnarep.2005.04.001.
The multiple BRCT-domain protein TopBP1 and its yeast homologs have been implicated in many aspects of DNA metabolism, but their molecular functions remain elusive. In this review, we first summarise how the yeast homologs were identified and characterised. We next review the data available from metazoan systems and finally draw parallels with the yeast models. TopBP1 plays important functions in the initiation of DNA replication in all organisms and participates in checkpoint responses both within S phase and following DNA damage. In metazoan systems there is accumulating evidence for additional roles in transcriptional regulation that have not been reported in yeast. Overall, TopBP1 appears to play a key role in integrating different aspects of DNA metabolism, but the mechanistic basis for this remains to be fully explained.
具有多个BRCT结构域的蛋白质TopBP1及其酵母同源物参与了DNA代谢的多个方面，但其分子功能仍不清楚。在本综述中，我们首先总结了酵母同源物是如何被鉴定和表征的。接下来，我们回顾了后生动物系统中的现有数据，最后将其与酵母模型进行比较。TopBP1在所有生物体的DNA复制起始中发挥重要作用，并参与S期内和DNA损伤后的检查点反应。在后生动物系统中，越来越多的证据表明它在转录调控中具有酵母中未报道的其他作用。总体而言，TopBP1似乎在整合DNA代谢的不同方面发挥关键作用，但其机制基础仍有待充分解释。
