Wang Yuanyuan, Wang Xiaolan, Yuan Hongyu, Chen Rongzhi, Zhu Lili, He Ruifeng, He Guangcun
Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan, P.R. China.
Mol Plant Microbe Interact. 2008 Jan;21(1):122-32. doi: 10.1094/MPMI-21-1-0122.
Rice (Oryza sativa L.) and brown planthoppers (BPH) (Nilaparvata lugens Stål) provide an ideal system for studying molecular mechanisms involved in the interactions between plants and phloem-feeding insects. The phenotypic responses and changes in transcript profiles of seedlings representing two rice cultivars differing in resistance to the BPH were analyzed. In the BPH-compatible (susceptible) cv. MH63, BPH feeding reduced three examined plant growth parameters (leaf area expansion, height increases, and dry weight increases) and photosynthetic rates of the leaves. In the BPH-incompatible (resistant) cv. B5, BPH feeding caused slight reductions in protein and sucrose contents, but the plants maintained their photosynthetic activity and grew normally. A cDNA microarray containing 1,920 suppression subtractive hybridization clones was used to explore the transcript profiles differences in the two cultivars under control and BPH-feeding conditions. In total, 160 unique genes were detected as being significantly affected by BPH feeding in rice plants, covering a wide range of functional categories, and there were 38 genes that showed the similar transcript pattern in both genotypes. The physiological responses and transcript profiles of plants represented in both genotypes suggested that multiple pathways might be involved in reprogramming of BPH-infested rice plants. The differences in transcript levels between the compatible and incompatible interactions revealed in this study were not only the reaction of resistance and susceptibility but also reflections of different damage rates and genotypic backgrounds of the rice cultivars.
水稻（Oryza sativa L.）和褐飞虱（Nilaparvata lugens Stål）为研究植物与吸食韧皮部昆虫之间相互作用所涉及的分子机制提供了一个理想的系统。分析了代表两个对褐飞虱抗性不同的水稻品种的幼苗的表型反应和转录谱变化。在与褐飞虱亲和（感虫）的品种MH63中，褐飞虱取食降低了三个检测的植物生长参数（叶面积扩展、株高增加和干重增加）以及叶片的光合速率。在与褐飞虱不亲和（抗性）的品种B5中，褐飞虱取食导致蛋白质和蔗糖含量略有降低，但植株保持了光合活性并正常生长。利用包含1920个抑制性消减杂交克隆的cDNA微阵列，探究了两个品种在对照和褐飞虱取食条件下的转录谱差异。总共检测到160个独特基因在水稻植株中受褐飞虱取食的显著影响，涵盖广泛的功能类别，并且有38个基因在两种基因型中呈现相似的转录模式。两种基因型植株的生理反应和转录谱表明，多个途径可能参与了受褐飞虱侵染的水稻植株的重编程。本研究揭示的亲和与不亲和相互作用之间转录水平的差异不仅是抗性和感病性的反应，也是水稻品种不同受害率和基因型背景的反映。
