Zwirglmaier Katrin
Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK.
FEMS Microbiol Lett. 2005 May 15;246(2):151-8. doi: 10.1016/j.femsle.2005.04.015.
Fluorescence in situ hybridisation (FISH) has become one of the major techniques in environmental microbiology. The original version of this technique often suffered from limited sensitivity due to low target copy number or target inaccessibility. In recent years there have been several developments to amend this problem by increasing signal intensity. This review summarises various approaches for signal amplification, focussing especially on two widely recognised varieties, tyramide signal amplification and multiply labelled polynucleotide probes. Furthermore, new applications for FISH are discussed, which arise from the increased sensitivity of the method.
荧光原位杂交（FISH）已成为环境微生物学中的主要技术之一。由于靶标拷贝数低或靶标难以接近，该技术的原始版本常常灵敏度有限。近年来，为改善这一问题，人们通过提高信号强度进行了多项改进。本综述总结了各种信号放大方法，尤其着重于两种广泛认可的类型，即酪胺信号放大和多重标记多核苷酸探针。此外，还讨论了因该方法灵敏度提高而产生的FISH新应用。
