Clark Amanda L, Slayden Ov D, Hettrich Kevin, Brenner Robert M
Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, USA.
Am J Obstet Gynecol. 2005 May;192(5):1523-9. doi: 10.1016/j.ajog.2004.11.042.
Our aim was to study the effect of estradiol and raloxifene on collagen synthesis, by measuring the expression collagen I and III mRNA.
Nineteen nulliparous young adult rhesus macaques underwent oophorectomy and were treated for 5 months with estradiol alone, raloxifene, or no hormone. Tissue samples were acquired from the lateral vaginal wall, and included the paravaginal attachment and levator ani muscle. Expression of mRNA for collagen I and III was measured by in situ hybridization.
Estradiol increased mRNA for collagen I and III compared with no hormone and raloxifene treatment (ANOVA, P < .05). Collagen mRNA was localized to fibroblasts in the vaginal connective tissue and the connective tissue investments of striated muscle. Collagen mRNA was not expressed in epithelial, smooth, and striated muscle cells.
Estrogen, but not raloxifene, increases collagen gene transcription and indicates stimulation of collagen synthesis in pelvic floor connective tissues.
我们的目的是通过测量I型和III型胶原蛋白mRNA的表达，研究雌二醇和雷洛昔芬对胶原蛋白合成的影响。
19只未生育的成年恒河猴接受了卵巢切除术，并分别用单独的雌二醇、雷洛昔芬或不用激素进行了5个月的治疗。从阴道侧壁获取组织样本，包括阴道旁附着组织和肛提肌。通过原位杂交测量I型和III型胶原蛋白的mRNA表达。
与不用激素和雷洛昔芬治疗相比，雌二醇增加了I型和III型胶原蛋白的mRNA（方差分析，P < .05）。胶原蛋白mRNA定位于阴道结缔组织中的成纤维细胞以及横纹肌的结缔组织中。上皮细胞、平滑肌细胞和横纹肌细胞中未表达胶原蛋白mRNA。
雌激素而非雷洛昔芬可增加胶原蛋白基因转录，并表明其刺激盆底结缔组织中的胶原蛋白合成。
