不可逆电通透化酵母保留了通过氧化磷酸化合成ATP的能力。

Irreversibly electropermeabilized yeast retains the capability for ATP synthesis via oxidative phosphorylation.

作者信息

Ganeva V, Galutzov B, Teissié J

机构信息

Faculty of Biology, University of Sofia, 8 blvd Dragan Tzankov, 1164 Sofia, Bulgaria.

出版信息

Bioelectrochemistry. 2006 Jan;68(1):27-30. doi: 10.1016/j.bioelechem.2005.01.003.

DOI:10.1016/j.bioelechem.2005.01.003

PMID:15905136

Abstract

ATP synthesis in irreversibly electropermeabilized yeast Kluyveromyces lactis was studied by using different respiratory substrates. The permeabilization itself provoked a dramatic decrease of the total ATP level and the cells lost their ability to synthesize ATP via glycolysis. The addition of exogenous NADH supported ATP synthesis in irreversibly permeabilized cells for up to 4-6 h after substrate addition when the total ATP level became twice that of intact cells incubated for the same period with lactose.

摘要

利用不同的呼吸底物研究了不可逆电通透化的乳酸克鲁维酵母中的ATP合成。通透化本身导致总ATP水平急剧下降，细胞失去了通过糖酵解合成ATP的能力。添加外源NADH后，不可逆通透化细胞中的ATP合成可维持4 - 6小时，此时总ATP水平是与乳糖孵育相同时间的完整细胞的两倍。