Kitagawa T
J Cell Physiol. 1980 Jan;102(1):37-43. doi: 10.1002/jcp.1041020106.
Exogenous ATP has been shown to cause a rapid and reversible increase in permeability in transformed 3T3 cells (3T6 and SV3T3) but not in untransformed 3T3 cells. The cells remain viable, but lose intracellular acid-soluble pools. Treatment of transformed cells with ATP greatly reduces incorporation of 14C-leucine into protein, which is restored by the incubation of the cells with Dulbecco's modified Eagle's medium or by the external additions of certain ions and energy sources. tRNA is not required for the restoration of protein synthesis. In the permeabilized cells the energy for protein synthesis can be provided by glycolysis, oxidative phosphorylation, or direct addition of ATP. These studies demonstrate the usefulness of this method for studying the control of metabolism and macromolecular synthesis in monolayer cultures of transformed mammalian cells.
外源性ATP已被证明可使转化的3T3细胞（3T6和SV3T3）的通透性迅速且可逆地增加，但未转化的3T3细胞则不会。细胞仍保持活力，但失去细胞内酸溶性池。用ATP处理转化细胞会大大降低14C-亮氨酸掺入蛋白质的量，通过将细胞与杜氏改良伊格尔培养基一起孵育或通过外部添加某些离子和能量来源可恢复该掺入量。蛋白质合成的恢复不需要tRNA。在通透细胞中，蛋白质合成所需的能量可由糖酵解、氧化磷酸化或直接添加ATP提供。这些研究证明了该方法在研究转化哺乳动物细胞单层培养物中代谢控制和大分子合成方面的有用性。
