Makan N R, Heppel L A
J Cell Physiol. 1978 Jul;96(1):87-94. doi: 10.1002/jcp.1040960111.
Exogenous ATP has been shown earlier to activate a permeability change in transformed 3T3 cultures leading to massive efflux of the acid-soluble pools. This leads to reduction of the basal rate of glycolysis to a very low level so that glycolysis becomes almost totally dependent on the addition to the medium of glucose, inorganic phosphate and ADP in order to restore the rate to that of untreated cells. No such depression of glycolysis is observed in untreated transformed cells or in ATP-treated normal 3T3 cells. In such permeabilized cultures, phosphorylated intermediates such as glucose-6-phosphate and fructose-1,6-diphosphate can serve as effective substrates for lactic acid formation. ATP treatment of cultured cells also allows molecules as big as NADP to enter the cells and participate in the pentose phosphate shunt pathway. This ability to temporarily and differentially render transformed cells permeable allows a review of several aspects of cellular metabolism and biosynthesis in the intact cell where the cellular organization is maintained. Furthermore, it deserves serious consideration as a means to achieve differential cytotoxicity of transformed cells by chemotherapeutic agents which, on their own, are indiscriminate in their action.
外源ATP早前已被证明可激活转化的3T3培养物中的通透性变化，导致酸溶性池大量外流。这使得糖酵解的基础速率降低到非常低的水平，以至于糖酵解几乎完全依赖于向培养基中添加葡萄糖、无机磷酸盐和ADP，以便将速率恢复到未处理细胞的水平。在未处理的转化细胞或ATP处理的正常3T3细胞中未观察到这种糖酵解抑制现象。在这种通透化的培养物中，磷酸化中间体如葡萄糖-6-磷酸和果糖-1,6-二磷酸可作为乳酸形成的有效底物。对培养细胞进行ATP处理还能使像NADP这么大的分子进入细胞并参与磷酸戊糖途径。这种使转化细胞暂时且有差异地通透的能力，使得在维持细胞组织结构的完整细胞中，可以对细胞代谢和生物合成的几个方面进行研究。此外，作为一种通过化疗药物实现对转化细胞的差异细胞毒性的手段，它值得认真考虑，因为化疗药物本身的作用是无差别的。
