Boucrot Emmanuel, Henry Thomas, Borg Jean-Paul, Gorvel Jean-Pierre, Méresse Stéphane
Centre d'Immunologie de Marseille-Luminy, CNRS-INSERM-Université de la Méditerranée, Parc Scientifique de Luminy, Case 906-13288 Marseille Cedex 9, France.
Science. 2005 May 20;308(5725):1174-8. doi: 10.1126/science.1110225.
Salmonella enterica causes a variety of diseases, including gastroenteritis and typhoid fever. The success of this pathogen depends on its capacity to proliferate within host cells in a membrane-bound compartment. We found that the Salmonella-containing vacuole recruited the plus-end-directed motor kinesin. Bacterial effector proteins translocated into the host cell by a type III secretion system antagonistically regulated this event. Among these effectors, SifA targeted SKIP, a host protein that down-regulated the recruitment of kinesin on the bacterial vacuole and, in turn, controlled vacuolar membrane dynamics.
肠炎沙门氏菌会引发多种疾病，包括肠胃炎和伤寒热。这种病原体的成功传播取决于其在宿主细胞膜结合区室中增殖的能力。我们发现，含沙门氏菌的液泡招募了正端定向运动蛋白驱动蛋白。通过III型分泌系统转运到宿主细胞中的细菌效应蛋白对这一过程进行了拮抗调节。在这些效应蛋白中，SifA靶向SKIP，SKIP是一种宿主蛋白，它下调了驱动蛋白在细菌液泡上的募集，进而控制液泡膜动力学。
