Tsuji S, Kawano S, Michida T, Masuda E, Nagano K, Takei Y, Fusamoto H, Kamada T
Department of Medicine, Osaka University Medical School, Japan.
Alcohol Clin Exp Res. 1992 Apr;16(2):347-9. doi: 10.1111/j.1530-0277.1992.tb01389.x.
The present study employed enzyme-immunoassay to examine the effect of ethanol on endothelin-1 and/or -2(ET1 + 2) release from human umbilical vein endothelial cells. Thirty minutes of exposure to ethanol increased the release of immunoreactive ET1 + 2 from cultured endothelial cells in a dose-dependent manner. However, ethanol at concentrations of less than 400 mM did not induce any LDH release from the endothelial cells. Trypan blue exclusion test revealed that 400 mM solution of ethanol decreased the cell viability to 7.7%. Thus, ethanol was found to directly stimulate ET1 + 2 release from cultured human umbilical vein endothelial cells. This reaction of vascular endothelial cells against ethanol may be related to ethanol-induced cardiovascular diseases such as hypertension, myocardial infarction and stroke, as well as fatal alcohol syndrome.
本研究采用酶免疫分析法检测乙醇对人脐静脉内皮细胞释放内皮素-1和/或-2（ET1 + 2）的影响。暴露于乙醇30分钟后，培养的内皮细胞中免疫反应性ET1 + 2的释放呈剂量依赖性增加。然而，浓度低于400 mM的乙醇未诱导内皮细胞释放任何乳酸脱氢酶。台盼蓝排斥试验显示，400 mM乙醇溶液可使细胞活力降至7.7%。因此，发现乙醇可直接刺激培养的人脐静脉内皮细胞释放ET1 + 2。血管内皮细胞对乙醇的这种反应可能与乙醇诱导的心血管疾病有关，如高血压、心肌梗死和中风，以及致命性酒精综合征。
