Hernández-Ochoa Isabel, García-Vargas Gonzalo, López-Carrillo Lizbeth, Rubio-Andrade Marisela, Morán-Martínez Javier, Cebrián Mariano E, Quintanilla-Vega Betzabet
Sección Externa de Toxicología, CINVESTAV-IPN, Ave. IPN #2508, Colonia Zacatenco, México D.F. 07360, Mexico.
Reprod Toxicol. 2005 Jul-Aug;20(2):221-8. doi: 10.1016/j.reprotox.2005.01.007.
We evaluated environmental-lead (Pb) effects on semen quality and sperm chromatin, considering Pb in seminal fluid (PbSF), spermatozoa (PbSpz), and blood (PbB) as exposure biomarkers in urban men (9.3 microg/dL PbB). Several individuals (44%) showed decreases in sperm quality; sperm concentration, motility, morphology and viability associated negatively with PbSpz, whereas semen volume associated negatively with PbSF. Multiple linear regression estimated PbSF and PbSpz thresholds for alterations in semen quality. Forty-eight percent of samples showed high values of nuclear chromatin condensation (NCD) positively associated with PbSF and zinc in spermatozoa (ZnSpz). ZnSpz values were higher than in fertile men. These results suggest that Pb may affect sperm chromatin by altering sperm Zn availability. PbB was not associated with semen quality or NCD, suggesting that Pb in semen compartments assesses better the amount of Pb in the reproductive tract; therefore, these are better biomarkers to evaluate toxicity at low Pb-exposure levels.
我们评估了环境铅（Pb）对精液质量和精子染色质的影响，将精液中的铅（PbSF）、精子中的铅（PbSpz）和血液中的铅（PbB）视为城市男性（血铅水平为9.3微克/分升）的暴露生物标志物。部分个体（44%）精液质量下降；精子浓度、活力、形态和存活率与PbSpz呈负相关，而精液体积与PbSF呈负相关。多元线性回归估计了精液质量改变时的PbSF和PbSpz阈值。48%的样本显示核染色质凝聚（NCD）值较高，且与精子中的PbSF和锌（ZnSpz）呈正相关。ZnSpz值高于生育能力正常的男性。这些结果表明，铅可能通过改变精子中锌的可利用性来影响精子染色质。血铅水平与精液质量或NCD无关，这表明精液成分中的铅能更好地评估生殖道中的铅含量；因此，在低铅暴露水平下，这些是评估毒性的更好生物标志物。
