Tao R V, Kovathana N, Shen Y W
Curr Eye Res. 1982;2(7):427-34. doi: 10.3109/02713688208996345.
Five neutral glycosphingolipids were isolated from human cataracts using silicic acid column chromatography and preparative thin-layer chromatography. Three of these glycolipids were partially identified by gas-liquid chromatography as glucosylceramide, lactosylceramide, and trihexosylceramide. Two glucosamine-containing glycosphingolipids (one of which contained fucose) were also detected. One of these two lipids contained galactose, glucose, N-acetylglucosamine in the molar ratio of 2:1:1, while the other contained fucose, galactose, glucose, and N-acetylglucosamine with the molar ratio of 1:2:1:1. Dihydrosphingosine (sphinganine) was the major long-chain base detected in all these fractions. The fatty acids of these neutral glycosphingolipids were variable in chain length, although the majority of them were greater than 20 carbons. This represents the first time whereby a family of neutral glycosphingolipids has been detected in human cataracts. This is also the first demonstration of the existence of a neutral fucolipid in the lenses of any species.
利用硅酸柱色谱法和制备型薄层色谱法从人白内障中分离出5种中性糖鞘脂。其中3种糖脂通过气液色谱法被部分鉴定为葡糖神经酰胺、乳糖神经酰胺和三己糖神经酰胺。还检测到两种含葡糖胺的糖鞘脂（其中一种含有岩藻糖）。这两种脂质中的一种含有摩尔比为2:1:1的半乳糖、葡萄糖、N-乙酰葡糖胺，而另一种含有摩尔比为1:2:1:1的岩藻糖、半乳糖、葡萄糖和N-乙酰葡糖胺。二氢鞘氨醇（鞘氨醇）是在所有这些组分中检测到的主要长链碱。这些中性糖鞘脂的脂肪酸链长度各不相同，不过其中大多数碳链长度大于20个碳。这是首次在人白内障中检测到一类中性糖鞘脂。这也是首次证明在任何物种的晶状体中存在中性岩藻糖脂。
