Llamas Inmaculada, Quesada Emilia, Martínez-Cánovas Maria José, Gronquist Matthew, Eberhard Anatol, González Juan E
Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX 75083-0688, USA.
Extremophiles. 2005 Aug;9(4):333-41. doi: 10.1007/s00792-005-0448-1. Epub 2005 May 21.
Some members of the moderately halophilic genus Halomonas, such as H. eurihalina, H. maura, H. ventosae and H. anticariensis, produce exopolysaccharides with applications in many industrial fields. We report here that these four species also produce autoinducer molecules that are involved in the cell-to-cell signaling process known as quorum sensing. By using the N-acyl homoserine lactone (AHL) indicator strains Agrobacterium tumefaciens NTL4 (pZRL4) and Chromobacterium violaceum CV026, we discovered that all the Halomonas strains examined synthesize detectable AHL signal molecules. The synthesis of these compounds was growth-phase dependent and maximal activity was reached during the late exponential to stationary phases. One of these AHLs seems to be synthesized only in the stationary phase. Some of the AHLs produced by H. anticariens FP35(T) were identified by gas chromatography/mass spectrometry and electrospray ionization tandem mass spectrometry as N-butanoyl homoserine lactone (C(4)-HL), N-hexanoyl homoserine lactone (C(6)-HL), N-octanoyl homoserine lactone (C(8)-HL) and N-dodecanoyl homoserine lactone (C(12)-HL). This study suggests that quorum sensing may also play an important role in extreme environments.
中度嗜盐的嗜盐单胞菌属（Halomonas）的一些成员，如嗜盐栖居嗜盐单胞菌（H. eurihalina）、莫拉嗜盐单胞菌（H. maura）、通风嗜盐单胞菌（H. ventosae）和抗龋嗜盐单胞菌（H. anticariensis），能产生胞外多糖，在许多工业领域有应用。我们在此报告，这四个物种还能产生自诱导分子，这些分子参与了被称为群体感应的细胞间信号传导过程。通过使用N-酰基高丝氨酸内酯（AHL）指示菌株根癌农杆菌NTL4（pZRL4）和紫色杆菌CV026，我们发现所有检测的嗜盐单胞菌菌株都能合成可检测到的AHL信号分子。这些化合物的合成依赖于生长阶段，在指数后期到稳定期达到最大活性。其中一种AHL似乎只在稳定期合成。抗龋嗜盐单胞菌FP35(T)产生的一些AHL通过气相色谱/质谱联用和电喷雾电离串联质谱法被鉴定为N-丁酰高丝氨酸内酯（C(4)-HL）、N-己酰高丝氨酸内酯（C(6)-HL）、N-辛酰高丝氨酸内酯（C(8)-HL）和N-十二酰高丝氨酸内酯（C(12)-HL）。这项研究表明群体感应在极端环境中可能也起着重要作用。
