Scheffler S U, Scherler J, Pruss A, von Versen R, Weiler A
Sports Traumatology and Arthroscopy Service, Center for Musculoskeletal Surgery, University Hospital Charité, Berlin, Germany.
Cell Tissue Bank. 2005;6(2):109-15. doi: 10.1007/s10561-004-6403-z.
Recent reports of disease transmission following ACL reconstruction with fresh-frozen non-sterilized allografts have highlighted the need for new sterilization techniques that do not impair the mechanical properties as it was shown for most of the current sterilization techniques. In this in-vitro biomechanical study, it was investigated if peracetic acid ethanol sterilization (PES) has any adverse effects on the mechanical properties of human bone-patellar tendon-bone grafts (BPTB). Paired human BPTB grafts either underwent PES or were used as fresh-frozen non-sterilized grafts. Viscoelastic properties (strain, creep) were analyzed during cyclic submaximal loading and mechanical properties were investigated during load-to-failure (LTF) testing. It was found that there were no differences in viscoelastic and mechanical properties between both groups. The findings of this study provide baseline data for future in vitro and in vivo analyses of this promising new sterilization technique for soft-tissue allografts.
近期有关使用新鲜冷冻的未灭菌同种异体移植物进行前交叉韧带重建术后疾病传播的报道凸显了对新灭菌技术的需求，这种新技术不会损害机械性能，而目前大多数灭菌技术都存在这样的问题。在这项体外生物力学研究中，研究了过氧乙酸乙醇灭菌（PES）对人骨-髌腱-骨移植物（BPTB）的机械性能是否有任何不良影响。将成对的人BPTB移植物要么进行PES处理，要么用作新鲜冷冻的未灭菌移植物。在循环次最大负荷期间分析粘弹性特性（应变、蠕变），并在破坏负荷（LTF）测试期间研究机械性能。结果发现两组之间的粘弹性和机械性能没有差异。本研究结果为未来对这种有前景的软组织同种异体移植物新灭菌技术进行体外和体内分析提供了基线数据。
