Kumar Varinder, Ganguly N k, Joshi Kusum, Mittal Rahul, Harjai Kusum, Chhibber Sanjay, Sharma Saroj
Department of Microbiology, Panjab University, Basic Medical Sciences Building, Sector 14, Chandigarh, 160014, India.
Med Microbiol Immunol. 2005 Aug;194(4):211-7. doi: 10.1007/s00430-005-0241-x. Epub 2005 May 21.
In the present study, protective efficacy of Escherichia coli capsular antigen, K13, was evaluated in a mouse model of pyelonephritis. Unconjugated capsular polysaccharide failed to provide any protection. However, coupling of K13 to diphtheria toxoid (DT) enhanced its immunogenicity and led to significant production of anticapsular antibodies in mice. Immunization of animals with K13-DT conjugate also caused significant improvement in cell-mediated immune response as indicated by an increase in lymphoblastogenic response and in the CD4+/CD8+ cell ratio of splenic lymphocytes. Significant decrease in bacterial load and renal severity scores were observed in K13-DT immunized animals. Suitability of K13-DT conjugate as an effective vaccine candidate against urinary tract infections caused by E. coli has been discussed.
在本研究中，在肾盂肾炎小鼠模型中评估了大肠杆菌荚膜抗原K13的保护效力。未结合的荚膜多糖未能提供任何保护作用。然而，将K13与白喉类毒素（DT）偶联可增强其免疫原性，并导致小鼠体内产生大量抗荚膜抗体。用K13-DT偶联物免疫动物还使细胞介导的免疫反应显著改善，表现为成淋巴细胞反应增加以及脾淋巴细胞CD4+/CD8+细胞比率升高。在接受K13-DT免疫的动物中观察到细菌载量和肾脏严重程度评分显著降低。已讨论了K13-DT偶联物作为抗大肠杆菌引起的尿路感染的有效候选疫苗的适用性。
