Zhou Li-hua, Li Yu-qin, Li Yong-quan, Wu Dan
School of Life Science, Zhejiang University, Hangzhou 310027, China.
J Zhejiang Univ Sci B. 2005 Jun;6(6):464-9. doi: 10.1631/jzus.2005.B0464.
Confocal laser scanning microscopy was used to observe the spatio-temporal expression of the pathway-specific gene redD during S. coelicolor cell cultivation. The corresponding mutant S. coelicolor lyqRY1522 carrying redD::eyfp in the chromosome was constructed. The temporal expression results of the fusion protein during submerged cultivation demonstrated that expression of redD began in the transition phase, continuing through the exponential growth phase to the stationary phase, and reached maximum in the stationary phase. On the other hand, redD was expressed only in substrate mycelia during solid-state culture, while aerial mycelia remained essentially non-fluorescent throughout culture. Results demonstrated that the expression pattern of redD coincides with that of the biosynthesis of the antibiotics during culture, revealing a direct correlation between the spatio-temporal distribution of regulatory gene expression and second metabolism.
利用共聚焦激光扫描显微镜观察了天蓝色链霉菌细胞培养过程中途径特异性基因redD的时空表达。构建了在染色体上携带redD::eyfp的相应突变体天蓝色链霉菌lyqRY1522。融合蛋白在深层培养过程中的时间表达结果表明，redD的表达始于过渡期，持续到指数生长期至稳定期，并在稳定期达到最大值。另一方面，在固态培养过程中，redD仅在基内菌丝中表达，而气生菌丝在整个培养过程中基本无荧光。结果表明，redD的表达模式与培养过程中抗生素的生物合成模式一致，揭示了调控基因表达的时空分布与次生代谢之间的直接相关性。
