Relić Dubravka, Dordević Dragana, Popović Aleksandar, Blagojević Tamara
Faculty of Chemistry, University of Belgrade, P.O. Box 158, Belgrade 11001, Serbia and Montenegro.
Environ Int. 2005 Jul;31(5):661-9. doi: 10.1016/j.envint.2004.11.003. Epub 2004 Dec 24.
A sequential extraction procedure was applied to identify forms of Ni, Zn, Pb and Cu with Fe- and Mn-oxides associated in alluvial sediments of the River Danube within Pancevo Oil Refinery (Serbia). The five steps of the sequential extraction procedure partitioned metals into: CH(3)COONH(4) extractable (S1); NH(2)OH.HCl carbonate extractable and easily reducible (S2); (NH(4))(2)C(2)O(2)/H(2)C(2)O(2) moderately reducible (S3); H(2)O(2)-HNO(3) organic extractable (S4); and HCl acid soluble residue (S5). Extracted concentrations of trace metals, analyzed after all five steps, were found to be (mg kg(-1)) for Mn: 656, Fe: 26734, Ni: 32.3, Zn: 72.8, Pb: 13.4 and Cu: 27.0. Most of the elements were found in acid soluble residue, characterizing stable compounds in sediments. Non-residual fractions of trace metals (sum of the first four fractions) were analyzed because they are more bioavailable than the residual amount. Correlation analysis and two multivariate analysis methods (principal component and cluster analysis) were used to understand and visualize the associations between the non-residual fractions of trace metals and certain forms, more or less crystalline of Fe- and Mn-oxides within the analyzed sediments, since Fe- and Mn-oxides play an important role in trace metal sorption within aquatic systems, especially within the Danube alluvium where the fluctuations of groundwater are very frequent and the level of groundwater could come close to surface.
采用连续提取法来确定塞尔维亚潘切沃炼油厂内多瑙河冲积沉积物中与铁和锰氧化物结合的镍、锌、铅和铜的形态。连续提取法的五个步骤将金属分为：醋酸铵可提取态（S1）；盐酸羟胺碳酸盐可提取且易还原态（S2）；草酸铵/草酸中度还原态（S3）；过氧化氢-硝酸有机可提取态（S4）；以及盐酸酸溶残渣态（S5）。经过所有五个步骤后分析得出的痕量金属提取浓度（mg kg⁻¹）分别为：锰656、铁26734、镍32.3、锌72.8、铅13.4和铜27.0。大部分元素存在于酸溶残渣中，这表明沉积物中的化合物较为稳定。对痕量金属的非残留部分（前四个部分的总和）进行了分析，因为它们的生物有效性高于残留量。采用相关性分析和两种多元分析方法（主成分分析和聚类分析）来理解和可视化分析沉积物中痕量金属非残留部分与铁和锰氧化物的某些或多或少结晶形态之间的关联，因为铁和锰氧化物在水生系统尤其是多瑙河冲积层中的痕量金属吸附中起着重要作用，在多瑙河冲积层中，地下水波动频繁，地下水位可能接近地表。
