Korenkov M, Yuecel N, Koebke J, Schierholz J, Morsczeck Ch, Tasci I, Neugebauer E A M, Nagelschmidt M
Surgical Clinic, Department of Surgery, University of Mainz, Langenbeckstrasse 1, 55131 Mainz, Germany.
Hernia. 2005 Oct;9(3):252-8. doi: 10.1007/s10029-005-0341-y. Epub 2005 May 24.
The purpose of this study was to investigate different forms of the local application of TGF-beta(1) for augmentation of the anterior abdominal wall in an appropriate model of an incisional hernia. Sixty male Sprague-Dawley rats were divided into six groups. Artificial defects of the anterior abdominal wall were closed with one of the following methods: running Prolene suture, Vicryl mesh, prolene suture followed by an intramuscular injection of 1 mug TGF-beta(1), Vicryl mesh coated with 1 mug TGF-beta(1), and prolene suture coated with 1 mug TGF-beta(1). A control group did not receive any defect and treatment. Six weeks after operation, tensile strength, collagen content, gene expression of collagen I and III, blood vessels, and thickness of collagen fibres were evaluated. Tensile strength was strongest in the controls (14.2 (10.5-18 N)). There was no increase in tensile strength due to the administration of TGF-beta(1). On the contrary, bolus injection of the growth factor resulted in a significantly decreased strength of the wound tissue when compared to the groups 1, 4, 5, and 6 (9.1 (4.2-9.1 N)). These results correlated with the gene expression of collagen I and III. Local application of TGF-beta(1) did not augment the strength of the abdominal wall after 6 weeks.
本研究的目的是在合适的切口疝模型中，研究转化生长因子β1（TGF-β1）局部应用的不同形式对腹前壁增强的作用。60只雄性Sprague-Dawley大鼠被分为6组。采用以下方法之一闭合腹前壁的人工缺损：连续普理灵缝线缝合、薇乔网片修补、普理灵缝线缝合后肌内注射1微克TGF-β1、涂有1微克TGF-β1的薇乔网片修补、涂有1微克TGF-β1的普理灵缝线缝合。对照组不制造缺损也不进行治疗。术后6周，评估拉伸强度、胶原蛋白含量、I型和III型胶原蛋白的基因表达、血管以及胶原纤维厚度。对照组的拉伸强度最强（14.2（10.5 - 18牛））。TGF-β1给药后拉伸强度没有增加。相反，与第1、4、5和6组相比，生长因子的推注导致伤口组织强度显著降低（9.1（4.2 - 9.1牛））。这些结果与I型和III型胶原蛋白的基因表达相关。6周后，TGF-β1的局部应用并未增强腹壁强度。
