Trimnell Adama R, Davies Gillian M, Lissina Olga, Hails Rosie S, Nuttall Patricia A
Seattle Biomedical Research Institute, WA 98109-5219, USA.
Vaccine. 2005 Jul 29;23(34):4329-41. doi: 10.1016/j.vaccine.2005.03.041.
Truncated constructs of 64P (64TRPs), a secreted cement protein from salivary glands of the tick Rhipicephalus appendiculatus, provided cross-protection against Rhipicephalus sanguineus and Ixodes ricinus, apparently by targeting antigens in the midgut and salivary glands of adults and nymphs, causing mortality. Tick feeding on 64TRP-immunised animals stimulated local inflammatory immune responses (involving basophils, eosinophils, lymphocytes, mast cells, macrophages and dendritic-like cells) that boosted the immune status of vaccinated animals. The vaccine trial results, and antigenic cross-reactivity of 64TRPs with R. sanguineus, I. ricinus, Amblyomma variegatum and Boophilus microplus, indicate the potential of 64TRPs as a broad-spectrum anti-tick vaccine.
64P（64TRPs）是一种来自蜱虫血红扇头蜱唾液腺的分泌性黏合蛋白的截短构建体，它能提供针对血红扇头蜱和蓖麻硬蜱的交叉保护，显然是通过靶向成虫和若虫中肠及唾液腺中的抗原，从而导致死亡。以64TRP免疫动物为食的蜱虫会刺激局部炎症免疫反应（涉及嗜碱性粒细胞、嗜酸性粒细胞、淋巴细胞、肥大细胞、巨噬细胞和树突状细胞），从而增强接种疫苗动物的免疫状态。疫苗试验结果以及64TRPs与血红扇头蜱、蓖麻硬蜱、变异革蜱和微小牛蜱的抗原交叉反应性表明，64TRPs作为一种广谱抗蜱疫苗具有潜力。
