Lippi U, Bellavite P, Schinella M, Nicoli M
Laboratorio di Chimica Clinica ed Ematologia, Ospedale Civile Maggiore, Verona, Italy.
Int J Clin Lab Res. 1992;21(4):321-4. doi: 10.1007/BF02591671.
Suspensions of phorbol myristate acetate-activated polymorphonuclear leukocytes were analyzed with a Coulter Counter STKS hematological analyzer. Phorbol myristate acetate activation induced an increase in polymorphonuclear leukocyte volume and conductivity, while scatter was unchanged. Phorbol myristate acetate-activated neutrophils in a suspension containing nitroblue tetrazolium showed increased scatter. The rise in scatter was phorbol myristate acetate dose dependent, completely inhibited by diphenylene iodonium and partially by dimethyl sulfoxide, two inhibitors of NADPH oxidase. Zymosan-activated polymorphonuclear leukocytes were notably larger with a characteristic position on discriminant function 1 display (volume versus scatter) of the analyzer. Volume and conductivity changes were seemingly inexplicable features of phorbol myristate acetate activation. The rise in scatter was produced by cytoplasmic precipitation of reduced nitroblue tetrazolium and thus by O2-generation in phorbol myristate acetate-activated neutrophils. Zymosan phagocytosis was responsible for the notable rise in polymorphonuclear leukocyte volume. The analysis of activated polymorphonuclear leukocytes by Coulter Counter STKS may provide useful information on their activation and a pragmatic approach for studying function.
用库尔特计数器STKS血液分析仪分析佛波酯肉豆蔻酸酯乙酸酯激活的多形核白细胞悬液。佛波酯肉豆蔻酸酯乙酸酯激活导致多形核白细胞体积和电导率增加，而散射不变。在含有硝基蓝四氮唑的悬液中，佛波酯肉豆蔻酸酯乙酸酯激活的中性粒细胞散射增加。散射的增加呈佛波酯肉豆蔻酸酯乙酸酯剂量依赖性，被二苯碘鎓完全抑制，被二甲基亚砜部分抑制，这两种都是NADPH氧化酶的抑制剂。酵母聚糖激活的多形核白细胞明显更大，在分析仪的判别函数1显示（体积与散射）上有特征性位置。体积和电导率的变化似乎是佛波酯肉豆蔻酸酯乙酸酯激活难以解释的特征。散射的增加是由还原硝基蓝四氮唑的细胞质沉淀产生的，因此是由佛波酯肉豆蔻酸酯乙酸酯激活的中性粒细胞中O2的产生导致的。酵母聚糖吞噬导致多形核白细胞体积显著增加。用库尔特计数器STKS分析激活的多形核白细胞可能为其激活提供有用信息，并为研究功能提供一种实用方法。
