Shimokawa Yasushi, Hirata Ken-ichi, Ishida Tatsuro, Kojima Yoko, Inoue Nobutaka, Quertermous Thomas, Yokoyama Mitsuhiro
Division of Cardiovascular and Respiratory Medicine, Kobe University Graduate School of Medicine, Chuo-ku, Japan.
Cardiovasc Res. 2005 Jun 1;66(3):594-600. doi: 10.1016/j.cardiores.2005.01.013.
To gain a better understanding of the involvement of endothelial lipase (EL) in vascular disease, we examined whether the EL expression is regulated in animal models of hypertension.
The rat cDNA homologue of EL was identified using reverse transcription-polymerase chain reaction. Cultured rat aortic smooth muscle cells were stimulated with angiotensin II (Ang II) and phorbol 12-myristate 13-acetate (PMA), and EL mRNA expression was analyzed by Northern blotting. EL mRNA levels in tissues from stroke-prone spontaneously hypertensive rats (SHR-SP) and Ang II-induced hypertensive rats were evaluated using RNase protection assays.
Rat EL cDNA encoded a protein containing 493 amino acid residues including a signal peptide, and shares 91.9% and 80.9% sequence homology with murine and human EL, respectively. Northern blotting revealed that EL was expressed in a wide range of rat tissues. In cultured rat aortic smooth muscle cells, Ang II and PMA increased EL mRNA levels by 2.9- and 3.3-fold, respectively. In Ang II-induced hypertensive rats, EL expression was upregulated in the aorta, heart, and lung. In SHR-SP, EL expression was upregulated in the aorta and heart.
EL expression is increased in rat models of hypertension. Thus, EL might have a role in the local pathophysiology of vascular diseases.
为了更好地了解内皮脂肪酶（EL）在血管疾病中的作用，我们研究了在高血压动物模型中EL的表达是否受到调控。
使用逆转录-聚合酶链反应鉴定大鼠EL的cDNA同源物。用血管紧张素II（Ang II）和佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）刺激培养的大鼠主动脉平滑肌细胞，通过Northern印迹分析EL mRNA表达。使用核糖核酸酶保护试验评估易中风自发性高血压大鼠（SHR-SP）和Ang II诱导的高血压大鼠组织中的EL mRNA水平。
大鼠EL cDNA编码一种含有493个氨基酸残基的蛋白质，包括一个信号肽，与小鼠和人EL的序列同源性分别为91.9%和80.9%。Northern印迹显示EL在大鼠的多种组织中表达。在培养的大鼠主动脉平滑肌细胞中，Ang II和PMA分别使EL mRNA水平增加2.9倍和3.3倍。在Ang II诱导的高血压大鼠中，主动脉、心脏和肺中的EL表达上调。在SHR-SP中，主动脉和心脏中的EL表达上调。
在高血压大鼠模型中EL表达增加。因此，EL可能在血管疾病的局部病理生理学中起作用。
