未标记DNA序列插入枯草芽孢杆菌168基因组的多个位点：一种高效的筛选方法。

Insertion of unmarked DNA sequences in multiple loci of the Bacillus subtilis 168 genome: an efficient selection method.

作者信息

Uotsu-Tomita Rie, Kaneko Shinya, Tsuge Kenji, Itaya Mitsuhiro

机构信息

Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan.

出版信息

Biosci Biotechnol Biochem. 2005 May;69(5):1036-9. doi: 10.1271/bbb.69.1036.

DOI:10.1271/bbb.69.1036

PMID:15914929

Abstract

The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.

摘要

通过同时使用正选择方法，加速了具有多个突变的枯草芽孢杆菌菌株的构建过程。所有通过新霉素筛选出的菌株在不同基因组位点同时获得了两个突变。采用改进后的方法使得假阳性率极低。