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枯草芽孢杆菌染色体与携带同源DNA的质粒之间突变的便捷体内转移。

Facile in vivo transfer of mutations between the Bacillus subtilis chromosome and a plasmid harbouring homologous DNA.

作者信息

Chak K F, de Lencastre H, Liu H M, Piggot P J

出版信息

J Gen Microbiol. 1982 Nov;128(11):2813-6. doi: 10.1099/00221287-128-11-2813.

DOI:10.1099/00221287-128-11-2813
PMID:6818329
Abstract

Transformation of Bacillus subtilis strains carrying the point mutations spoIIA42 or spoIIA69 by a plasmid, pHM2, carrying the spoIIA+ allele gave occasional recombinants in which the plasmid had acquired the chromosomal mutation. Transformation of a spo+ strain by these derivative plasmids gave occasional recombinants in which the chromosome of the recipient had acquired the plasmid-borne spoIIA mutation. Both types of behaviour were recE+-dependent. Mutations spoIIA42 and spoIIA69 fell into a single complementation group.

摘要

携带点突变spoIIA42或spoIIA69的枯草芽孢杆菌菌株被携带spoIIA+等位基因的质粒pHM2转化,偶尔会产生重组体,其中质粒获得了染色体突变。用这些衍生质粒转化spo+菌株,偶尔会产生重组体,其中受体的染色体获得了质粒携带的spoIIA突变。这两种行为都依赖于recE+。突变spoIIA42和spoIIA69属于一个单一的互补群。

相似文献

1
Facile in vivo transfer of mutations between the Bacillus subtilis chromosome and a plasmid harbouring homologous DNA.枯草芽孢杆菌染色体与携带同源DNA的质粒之间突变的便捷体内转移。
J Gen Microbiol. 1982 Nov;128(11):2813-6. doi: 10.1099/00221287-128-11-2813.
2
Isolation and characterization of a recombinant plasmid carrying a functional part of the Bacillus subtilis spoIIA locus.携带枯草芽孢杆菌spoIIA基因座功能部分的重组质粒的分离与鉴定。
J Gen Microbiol. 1982 Nov;128(11):2805-12. doi: 10.1099/00221287-128-11-2805.
3
Use of the Escherichia coli transposon Tn1000 (gamma delta) to generate mutations in Bacillus subtilis DNA.利用大肠杆菌转座子Tn1000(γδ)在枯草芽孢杆菌DNA中产生突变。
J Gen Microbiol. 1983 Oct;129(10):3203-10. doi: 10.1099/00221287-129-10-3203.
4
[Plasmid transformation and transduction in different rec-mutants of Bacillus subtilis].[枯草芽孢杆菌不同rec-突变体中的质粒转化与转导]
Genetika. 1982 Mar;18(3):413-9.
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Use of integrational plasmid vectors to demonstrate the polycistronic nature of a transcriptional unit (spoIIA) required for sporulation of Bacillus subtilis.使用整合质粒载体来证明枯草芽孢杆菌芽孢形成所需转录单位(spoIIA)的多顺反子性质。
J Gen Microbiol. 1984 Aug;130(8):2123-36. doi: 10.1099/00221287-130-8-2123.
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[The induction of auxotrophic mutations for riboflavin by the integration of plasmid pLRS33 into the chromosome of Bacillus subtilis].[通过质粒pLRS33整合到枯草芽孢杆菌染色体中诱导核黄素营养缺陷型突变]
Genetika. 1988 Aug;24(8):1371-4.
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[Determination of the minimal length DNA homologous region required for plasmid integration into the Bacillus subtilis chromosome via homologous recombination].[通过同源重组将质粒整合到枯草芽孢杆菌染色体所需的最小长度DNA同源区域的测定]
Genetika. 1992 Jul;28(7):38-45.
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[Intraspecific and interspecific transmission of plasmid and chromosomal DNA during natural transformation in Bacillus subtilis and Escherichia coli].[枯草芽孢杆菌和大肠杆菌自然转化过程中质粒和染色体DNA的种内和种间传递]
Mikrobiologiia. 1990 Nov-Dec;59(6):1046-9.
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[Integration of various plasmids into the Bacillus subtilis chromosome].[各种质粒整合到枯草芽孢杆菌染色体中]
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[Plasmid and chromosomal gene transfer in Bacillus subtilis and Escherichia coli in natural transformation].[枯草芽孢杆菌和大肠杆菌自然转化中的质粒与染色体基因转移]
Mol Gen Mikrobiol Virusol. 1990 Sep(9):29-31.

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J Bacteriol. 1994 Jun;176(11):3102-10. doi: 10.1128/jb.176.11.3102-3110.1994.
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Mechanism of acquisition of chromosomal markers by plasmids in Haemophilus influenzae.流感嗜血杆菌中质粒获取染色体标记的机制。
J Bacteriol. 1984 Nov;160(2):662-7. doi: 10.1128/jb.160.2.662-667.1984.
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Catabolite-resistant sporulation (crsA) mutations in the Bacillus subtilis RNA polymerase sigma 43 gene (rpoD) can suppress and be suppressed by mutations in spo0 genes.枯草芽孢杆菌RNA聚合酶σ43基因(rpoD)中的抗分解代谢物芽孢形成(crsA)突变可被spo0基因突变抑制,同时也能抑制spo0基因突变。
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