Geldmacher Astrid, Skrastina Dace, Borisova Galina, Petrovskis Ivars, Krüger Detlev H, Pumpens Paul, Ulrich Rainer
Institute of Virology, Charité Medical School, Campus Mitte, D-10098 Berlin, Germany.
Vaccine. 2005 Jun 10;23(30):3973-83. doi: 10.1016/j.vaccine.2005.02.025. Epub 2005 Mar 16.
Hepatitis B virus (HBV) core particles carrying the amino-terminal 120 amino acids (aa) of the nucleocapsid (N) protein of the hantaviruses Dobrava, Hantaan or Puumala have been demonstrated to be highly immunogenic in mice when complexed with adjuvants. Here we demonstrate that even without adjuvant, these chimeric particles induced high-titered, and strongly cross-reactive N-specific antibody responses in BALB/c and C57BL/6 mice. The induced N-specific antibodies represented all IgG subclasses. Pre-existing core-specific antibodies did not abrogate the induction of an N-specific immune response by a hantavirus N insert presented on core particles. Therefore, chimeric core particles should represent promising vaccine candidates even for anti-core positive humans.
携带多布拉伐、汉坦或普马拉汉坦病毒核衣壳（N）蛋白氨基末端120个氨基酸（aa）的乙型肝炎病毒（HBV）核心颗粒，已被证明与佐剂复合时在小鼠中具有高度免疫原性。在此我们证明，即使没有佐剂，这些嵌合颗粒也能在BALB/c和C57BL/6小鼠中诱导高滴度且强烈交叉反应的N特异性抗体反应。诱导产生的N特异性抗体代表了所有IgG亚类。预先存在的核心特异性抗体并不会消除核心颗粒上呈现的汉坦病毒N插入片段对N特异性免疫反应的诱导。因此，嵌合核心颗粒即使对于抗核心阳性的人类也应是很有前景的疫苗候选物。
