Tyagi A, Santha I M, Mehta S L
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi.
Indian J Biochem Biophys. 1992 Feb;29(1):93-6.
Genomic DNA isolated from barley cv. NP 113 and its high lysine mutant Notch-2, and restricted with different restriction enzymes was hybridized with B1 and C-hordein DNA probes. Similar Southern hybridization patterns were observed between NP 113 and Notch-2. Dot blot hybridization analysis of RNA isolated at different developmental stages and from different tissues of seed showed temporal as well as tissue specific expression. The results obtained indicate that regulation at the level of transcription/post transcription may be responsible for lower accumulation of hordein in mutant Notch-2.
从大麦品种NP 113及其高赖氨酸突变体Notch-2中分离出的基因组DNA，用不同的限制性内切酶进行酶切后，与B1和C-醇溶蛋白DNA探针杂交。在NP 113和Notch-2之间观察到相似的Southern杂交模式。对在不同发育阶段以及从种子的不同组织中分离出的RNA进行斑点杂交分析，结果显示了其时间以及组织特异性表达。所获得的结果表明，转录/转录后水平的调控可能是突变体Notch-2中醇溶蛋白积累较低的原因。
