流动注射-化学发光检测法测定土霉素

Determination of oxytetracycline by flow injection with chemiluminescence detection.

作者信息

Guo Liangqia, Xie Zenghong, Bian Xintong, Lin Xucong, Zhang Weilin, Liu Xiaohua, Chen Guonan

机构信息

Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, People's Republic of China.

出版信息

Luminescence. 2005 May-Jun;20(3):129-34. doi: 10.1002/bio.827.

DOI:10.1002/bio.827

PMID:15924315

Abstract

A flow-injection chemiluminescent method for the determination of oxytetracycline was developed. The method is based on an enhancement by oxytetracycline of the chemiluminescence light emission of tris(2,2'-bipyridine) ruthenium (II), generated by the continuous oxidation of tris(2,2'-bipyridine) ruthenium (II) by cerium (IV) sulphate in sulphuric acid. Under the optimum conditions, the calibration curve was linear over the range 1.0 x 10(-7)-1.0 x 10(-5) g/mL for oxytetracycline with the linear equation: DeltaINT = 148.77 x C + 0.6637 (R2 = 0.9994). The detection limit was 4.52 x 10(-8) g/mL. The proposed method was also successfully used to determine oxytetracycline in pharmaceutical formulations. The mean recovery of determination of oxytetracycline was 92.73%. A mechanism for the chemiluminescence enhancement by oxytetracycline of tris(2,2'-bipyridine)-ruthenium (II) and cerium (IV) sulphate system is also proposed.

摘要

建立了一种流动注射化学发光法测定土霉素。该方法基于土霉素对硫酸铈（IV）在硫酸中连续氧化三（2,2'-联吡啶）钌（II）所产生的三（2,2'-联吡啶）钌（II）化学发光的增强作用。在最佳条件下，土霉素的校准曲线在1.0×10（-7）-1.0×10（-5）g/mL范围内呈线性，线性方程为：DeltaINT = 148.77×C + 0.6637（R2 = 0.9994）。检测限为4.52×10（-8）g/mL。该方法也成功用于药物制剂中土霉素的测定。土霉素测定的平均回收率为92.73%。还提出了土霉素增强三（2,2'-联吡啶）-钌（II）和硫酸铈（IV）体系化学发光的机理。