Lee Sang Hak, Li Ming, Suh Jung Kee
Department of Chemistry, Kyungpook National University, Taegu 702-701, Korea.
Anal Sci. 2003 Jun;19(6):903-6. doi: 10.2116/analsci.19.903.
A new chemiluminescence method for the determination of carbamazepine (CBZ) has been developed. The method is based on the chemiluminescence produced in the reaction of tris(2,2'-bipyridine)ruthenium(III) and CBZ in an acidic medium. The chemiluminescence intensity was enhanced by organic solvents in the reaction system. Under the optimum experimental conditions, the calibration curve was linear over the range 4.0 x 10(-3)-8.6 x 10(-7) mol/L for CBZ. The detection limit (S/N = 3) was 2.5 x 10(-7) mol/L and the relative standard deviation of six replicate measurements was 2.6% for 4.0 x 10(-4) mol/L of CBZ. The possible reaction mechanism were also discussed. The chemiluminescence method was successfully applied to assay the CBZ contents in pharmaceutical tablets.
已开发出一种用于测定卡马西平（CBZ）的新化学发光方法。该方法基于在酸性介质中三（2,2'-联吡啶）钌（III）与CBZ反应产生的化学发光。反应体系中的有机溶剂增强了化学发光强度。在最佳实验条件下，CBZ的校准曲线在4.0×10⁻³ - 8.6×10⁻⁷ mol/L范围内呈线性。检测限（S/N = 3）为2.5×10⁻⁷ mol/L，对于4.0×10⁻⁴ mol/L的CBZ，六次重复测量的相对标准偏差为2.6%。还讨论了可能的反应机理。该化学发光方法已成功应用于测定药片中CBZ的含量。
