流动注射化学发光法测定四环素：使用三（2,2'-联吡啶）钌（II）-硫酸铈（IV）体系

Flow injection chemiluminescent determination of tetracycline using a tris(2,2'-bipyridine)ruthenium(II)-cerium(IV) sulphate system.

作者信息

Guo Liangqia, Xie Zenghong, Lin Xucong, Liu Xiaohua, Zhang Weilin, Chen Guonan

机构信息

Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, People's Republic of China.

出版信息

Luminescence. 2004 Mar-Apr;19(2):64-8. doi: 10.1002/bio.757.

DOI:10.1002/bio.757

PMID:15098205

Abstract

A flow-injection chemiluminescence method for the determination of tetracycline was developed. The method is based on an enhancement by tetracycline of the chemiluminescence light emission of tris(2,2'-bipyridine)ruthenium(II). In sulphuric acid medium, the chemiluminescence is generated by the continuous oxidation of tris(2,2'-bipyridine)ruthenium(II) by cerium (IV) sulphate. The light-emission intensity is greatly enhanced in the presence of tetracycline. Under the optimum conditions, the calibration curve is linear over the range 3.75 x 10(-8) g/mL-1.5 x 10(-5) g/mL for tetracycline with the linear equation: deltaINT = 205.898 x C - 20.442 (R2 = 0.9974). The detection limit is 3.27 x 10(-8) g/mL. The proposed method was also successfully used to determine tetracycline in pharmaceutical formulation (mean recovery of tetracycline, 100.7%).

摘要

建立了一种流动注射化学发光法测定四环素。该方法基于四环素对三（2,2'-联吡啶）钌（II）化学发光的增强作用。在硫酸介质中，硫酸铈（IV）连续氧化三（2,2'-联吡啶）钌（II）产生化学发光。在四环素存在下，发光强度大大增强。在最佳条件下，四环素的校准曲线在3.75×10^(-8) g/mL - 1.5×10^(-5) g/mL范围内呈线性，线性方程为：ΔINT = 205.898×C - 20.442（R^2 = 0.9974）。检测限为3.27×10^(-8) g/mL。该方法还成功用于测定药物制剂中的四环素（四环素的平均回收率为100.7%）。