Guo Liangqia, Xie Zenghong, Lin Xucong, Liu Xiaohua, Zhang Weilin, Chen Guonan
Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, People's Republic of China.
Luminescence. 2004 Mar-Apr;19(2):64-8. doi: 10.1002/bio.757.
A flow-injection chemiluminescence method for the determination of tetracycline was developed. The method is based on an enhancement by tetracycline of the chemiluminescence light emission of tris(2,2'-bipyridine)ruthenium(II). In sulphuric acid medium, the chemiluminescence is generated by the continuous oxidation of tris(2,2'-bipyridine)ruthenium(II) by cerium (IV) sulphate. The light-emission intensity is greatly enhanced in the presence of tetracycline. Under the optimum conditions, the calibration curve is linear over the range 3.75 x 10(-8) g/mL-1.5 x 10(-5) g/mL for tetracycline with the linear equation: deltaINT = 205.898 x C - 20.442 (R2 = 0.9974). The detection limit is 3.27 x 10(-8) g/mL. The proposed method was also successfully used to determine tetracycline in pharmaceutical formulation (mean recovery of tetracycline, 100.7%).
建立了一种流动注射化学发光法测定四环素。该方法基于四环素对三（2,2'-联吡啶）钌（II）化学发光的增强作用。在硫酸介质中，硫酸铈（IV）连续氧化三（2,2'-联吡啶）钌（II）产生化学发光。在四环素存在下，发光强度大大增强。在最佳条件下，四环素的校准曲线在3.75×10^(-8) g/mL - 1.5×10^(-5) g/mL范围内呈线性，线性方程为：ΔINT = 205.898×C - 20.442（R^2 = 0.9974）。检测限为3.27×10^(-8) g/mL。该方法还成功用于测定药物制剂中的四环素（四环素的平均回收率为100.7%）。
