Yamamoto A, Keil L C, Reid I A
Department of Physiology, University of California, San Francisco 94143-0444.
Hypertension. 1992 Jun;19(6 Pt 2):799-803. doi: 10.1161/01.hyp.19.6.799.
Electrical stimulation of afferent renal nerves and activation of renal mechanoreceptors increase plasma vasopressin concentrations. In the present study, the effect of renal chemoreceptor activation on plasma vasopressin concentration was investigated in anesthetized rabbits. Renal chemoreceptors were activated with intrarenal infusions of bradykinin. With intrarenal infusion of bradykinin at 136 ng/min, plasma vasopressin concentration increased from 4.5 +/- 1.5 to 26.8 +/- 14.2 pg/ml at 5 minutes (p less than 0.01), whereas with infusion at 1,360 ng/min, plasma vasopressin increased from 5.9 +/- 2.0 to 54.4 +/- 16.4 pg/ml at 5 minutes (p less than 0.01). There was no significant change in plasma vasopressin during intravenous infusion of bradykinin at 136 ng/min. Infusion at 1,360 ng/min increased plasma vasopressin from 2.7 +/- 0.5 to 14.8 +/- 6.4 pg/ml (p less than 0.01), but this increase was significantly less than that produced by intrarenal infusion of the same dose of bradykinin. Similar effects on plasma vasopressin were observed during paired intrarenal and intravenous infusions of bradykinin at 136 ng/min. Renal denervation markedly reduced the vasopressin responses to intrarenal infusion of bradykinin at 136 ng/min (2.8 +/- 0.5 to 4.0 +/- 0.7 pg/ml, p less than 0.01) and 1,360 ng/min (3.2 +/- 0.7 to 7.8 +/- 1.8 pg/ml, p less than 0.05). These results indicate that bradykinin stimulates vasopressin release by an intrarenal action and suggest that this action is mediated by afferent renal nerves.
肾传入神经的电刺激以及肾机械感受器的激活会增加血浆血管加压素浓度。在本研究中，在麻醉兔身上研究了肾化学感受器激活对血浆血管加压素浓度的影响。通过肾内输注缓激肽激活肾化学感受器。肾内以136 ng/分钟的速度输注缓激肽时，5分钟时血浆血管加压素浓度从4.5±1.5 pg/ml增加到26.8±14.2 pg/ml（p<0.01），而以1360 ng/分钟的速度输注时，5分钟时血浆血管加压素从5.9±2.0 pg/ml增加到54.4±16.4 pg/ml（p<0.01）。以136 ng/分钟的速度静脉输注缓激肽期间，血浆血管加压素无显著变化。以1360 ng/分钟的速度输注会使血浆血管加压素从2.7±0.5 pg/ml增加到14.8±6.4 pg/ml（p<0.01），但这种增加明显小于相同剂量缓激肽肾内输注所产生的增加。在肾内和静脉配对输注136 ng/分钟缓激肽期间，观察到对血浆血管加压素有类似影响。肾去神经支配显著降低了对136 ng/分钟（从2.8±0.5 pg/ml到4.0±0.7 pg/ml，p<0.01）和1360 ng/分钟（从3.2±0.7 pg/ml到7.8±1.8 pg/ml，p<0.05）肾内输注缓激肽的血管加压素反应。这些结果表明，缓激肽通过肾内作用刺激血管加压素释放，并提示该作用由肾传入神经介导。
