Xu Guo-Ying, Hughes Michael G, Zhang Liping, Cain Lisa, McAdoo David J
Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1043, USA.
Neurosci Lett. 2005 Aug 26;384(3):271-6. doi: 10.1016/j.neulet.2005.04.100.
In vivo experiments addressing the role of released glutamate in damage caused by neurotrauma seldom administer glutamate itself because it usually produces relatively little damage when administered into central nervous system (CNS) tissue in vivo. However, because of recent observations that glutamate administered into the spinal cord at the levels attained following spinal cord injury (SCI) kills neurons and oligodendrocytes, we tested the effects of administering glutamate at those concentrations on locomotor function. The Basso-Beattie-Bresnahan (BBB) test and activity box measures demonstrated that those glutamate concentrations produce lasting functional impairments. Several parameters provided by the activity box provided sensitive measures of the degree of post-SCI impairment, demonstrating their substantial potential for evaluating outcomes of SCI. Results obtained also enhance evidence that glutamate toxicity contributes to secondary damage following SCI and suggest that damage to white matter is an important contributor to such damage.
针对释放的谷氨酸在神经创伤所致损伤中作用的体内实验很少直接给予谷氨酸本身，因为在体内将其注入中枢神经系统（CNS）组织时，通常只会产生相对较小的损伤。然而，由于最近有观察发现，在脊髓损伤（SCI）后达到的水平将谷氨酸注入脊髓会杀死神经元和少突胶质细胞，我们测试了以这些浓度给予谷氨酸对运动功能的影响。Basso-Beattie-Bresnahan（BBB）测试和活动箱测量表明，这些谷氨酸浓度会产生持久的功能障碍。活动箱提供的几个参数对SCI后损伤程度提供了敏感的测量，证明了它们在评估SCI结果方面的巨大潜力。所获得的结果也进一步证明谷氨酸毒性促成了SCI后的继发性损伤，并表明白质损伤是这种损伤的一个重要因素。
