Lugo Joel, Harkema Jack R, deFeijter-Rupp Heather, Bartner Lisa, Boruta Daniel, Robinson N Edward
Pulmonary Laboratory, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48823, USA.
Vet J. 2006 Sep;172(2):293-301. doi: 10.1016/j.tvjl.2005.04.018. Epub 2005 May 31.
This study was performed to determine if a peripheral sample of lung from the site where biopsy is conducted is representative of the rest of the lung and to investigate the relationship between airway inflammation and intraepithelial mucous production in the peripheral airways. Lung parenchyma samples were collected from five different regions of the lung in five control and five heaves-affected horses. Horse groups were defined by clinical response to stabling. Tissue sections were used for semi-quantitative scoring of lesions, to count the number of airways, to quantify the amount of stored mucosubstances (Vs) within the epithelium, and to count the number of epithelial cells in terminal airways. No significant differences were found between lung regions or between groups of horses. Lack of regional differences in airway structures means that a biopsy sample can be used for diagnosis and investigation of diffusely distributed diseases. Airway inflammation was correlated with mucous cell metaplasia and Vs. Therefore, in horses, mucus accumulation is partly caused by increased number of mucous cells and is associated with airway inflammation. Therapy targeted to reduce airway inflammation will help reduce the excessive mucous accumulation in horses.
本研究旨在确定取自活检部位的肺外周样本是否能代表肺的其他部分，并研究外周气道中气道炎症与上皮内黏液产生之间的关系。从五匹对照马和五匹患慢性阻塞性肺病的马的肺的五个不同区域采集肺实质样本。马的分组根据对圈养的临床反应来定义。组织切片用于病变的半定量评分、气道计数、上皮内储存的黏液物质（Vs）的定量以及终末气道上皮细胞计数。在肺区域之间或马的组间未发现显著差异。气道结构缺乏区域差异意味着活检样本可用于诊断和研究弥漫性分布的疾病。气道炎症与黏液细胞化生和Vs相关。因此，在马中，黏液积聚部分是由黏液细胞数量增加引起的，并且与气道炎症有关。旨在减轻气道炎症的治疗将有助于减少马体内过多的黏液积聚。
