Gotoh N, Tanaka S, Nishino T
Department of Microbiology, Kyoto Pharmaceutical University, Japan.
J Antimicrob Chemother. 1992 Mar;29(3):279-85. doi: 10.1093/jac/29.3.279.
The susceptibility of ten clinical isolates and a standard reference strain, ATCC25238, of Moraxella catarrhalis to 22 beta-lactam antibiotics was examined and compared with that of Escherichia coli strain B. All the strains of M. catarrhalis tested, especially the non beta-lactamase-producing strain ATCC25238, were more susceptible to a rang of structurally unrelated beta-lactam antibiotics, including small Mr carbapenems, than E. coli B. The permeability of the M. catarrhalis outer membrane to beta-lactam antibiotics was examined by the swelling technique with proteoliposomes reconstituted from outer membranes. The diffusion rate of beta-lactams through the liposome membrane was inversely related to their Mr, a relationship which might be expected for entry by diffusion through a porin.
检测了10株卡他莫拉菌临床分离株和1株标准参考菌株ATCC25238对22种β-内酰胺类抗生素的敏感性，并与大肠杆菌B菌株进行了比较。所有测试的卡他莫拉菌菌株，尤其是不产β-内酰胺酶的菌株ATCC25238，比大肠杆菌B对一系列结构不相关的β-内酰胺类抗生素更敏感，包括小分子碳青霉烯类。采用外膜重构的蛋白脂质体通过肿胀技术检测了卡他莫拉菌外膜对β-内酰胺类抗生素的通透性。β-内酰胺类药物通过脂质体膜的扩散速率与其分子量成反比，这种关系可能是通过孔蛋白扩散进入所预期的。
