大肠杆菌中乳糖操纵子与ilv基因簇之间融合的特性：ilvC-乳糖融合体
Characterization of fusions between the lac operon and the ilv gene cluster in Escherichia coli: ilvC-lac fusions.
作者信息
Smith J M, Umbarger H E
出版信息
J Bacteriol. 1977 Dec;132(3):870-5. doi: 10.1128/jb.132.3.870-875.1977.
Abstract
By means of the general procedure of Casadaban (J. Mol. Biol. 104: 541-556, 1976), the lac genes carried on a lambda-Mu-1 hybrid phage were inserted into a temperature-inducible Mu-1 prophage that had earlier been inserted into a site near the beginning of the ilvC gene of Escherichia coli strain K-12. Selection of temperature-resistant derivatives of the lysogen resulted in a fusion of the lac genes to a region of deoxyribonucleic acid that is transcribed under the control of the ilvC regulatory elements. A strain bearing the fusion was shown to be inducible for beta-galactosidase by acetohydroxybutyrate, a natural inducer of acetohydroxy acid isomeroreductase. Induction of the lysogen by mitomycin C led to the isolation of a plaque-forming lambda derivative carrying this ilvC-lac fusion.
摘要
通过卡萨达班的通用方法（《分子生物学杂志》104: 541 - 556, 1976），将携带在λ - Mu - 1杂交噬菌体上的乳糖操纵子基因插入到一个温度诱导型Mu - 1原噬菌体中，该原噬菌体先前已插入到大肠杆菌K - 12菌株ilvC基因起始位点附近的一个位点。对溶原菌的耐温衍生物进行筛选，结果使乳糖操纵子基因与一段在ilvC调控元件控制下转录的脱氧核糖核酸区域发生融合。携带这种融合体的菌株被证明可被乙酰羟丁酸（乙酰羟酸异构还原酶的天然诱导物）诱导产生β - 半乳糖苷酶。用丝裂霉素C诱导溶原菌，导致分离出携带这种ilvC - 乳糖操纵子融合体的形成噬菌斑的λ衍生物。
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