利用携带启动子 - lac融合的λ噬菌体的Mu d1（lac Apr）溶原菌构建：λ ppheA - lac的分离
Construction from Mu d1 (lac Apr) lysogens of lambda bacteriophage bearing promoter-lac fusions: isolation of lambda ppheA-lac.
作者信息
Gowrishankar J, Pittard J
出版信息
J Bacteriol. 1982 Jun;150(3):1122-9. doi: 10.1128/jb.150.3.1122-1129.1982.
Abstract
Bacteriophage Mu d1 (lac Aprr) was used to obtain strains of Escherichia coli K-12 in which the lac genes are expressed from the promoter of pheA, the structural gene for the enzyme chorismate mutase P-prephenate-dehydratase. A derivative of bacteriophage lambda which carries the pheA-lac fusion was prepared; the method used is generally applicable for the construction, from Mu dl lysogens, of specialized transducing lambda phage carrying the promoter-lac fusions. A restriction enzyme cleavage map of lambda ppheA-lac for the enzymes HindIII and PstI is presented.
摘要
噬菌体Mu d1（lac Aprr）被用于获得大肠杆菌K - 12菌株，其中lac基因由分支酸变位酶P - 预苯酸脱水酶的结构基因pheA的启动子表达。制备了携带pheA - lac融合体的λ噬菌体衍生物；所使用的方法通常适用于从Mu dl溶源菌构建携带启动子 - lac融合体的特异性转导λ噬菌体。给出了λ ppheA - lac对于限制性内切酶HindIII和PstI的酶切图谱。
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