Silva S S, Santos M I, Coutinho O P, Mano J F, Reis R L
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Campus de Gualtar, 4710-057, Braga, Portugal.
J Mater Sci Mater Med. 2005 Jun;16(6):575-9. doi: 10.1007/s10856-005-0534-z.
Blends of polysaccharides and proteins are a source for the development of novel materials with interesting and tailorable properties, with potential to be used in a range of biomedical applications. in this work a series of blended membranes composed by chitosan and soy protein isolate was prepared by solvent casting methodology. in addition, cross-linking was performed in situ with glutaraldehyde solutions in the range 5x10(-3)-0.1 M. Furthermore, the influence of the composition and cross-linking on the degradation behaviour, water uptake and cell adhesion was investigated. The obtained results showed that the incorporation of chitosan, associated to network formation by cross linking, promoted a slight decrease of water absorption and a slower degradability of the membranes. Moreover, direct contact biocompatibility studies, with L929 cells, indicate that the cross-linking enhances the capability of the material to support cell growth.
多糖和蛋白质的混合物是开发具有有趣且可定制特性的新型材料的来源，有潜力用于一系列生物医学应用。在这项工作中，通过溶剂浇铸法制备了一系列由壳聚糖和大豆分离蛋白组成的混合膜。此外，用浓度范围为5×10⁻³ - 0.1 M的戊二醛溶液进行原位交联。此外，研究了组成和交联对降解行为、吸水性和细胞粘附的影响。所得结果表明，壳聚糖的加入，与交联形成网络有关，促进了膜吸水性的轻微降低和降解速度的减慢。此外，与L929细胞的直接接触生物相容性研究表明，交联增强了材料支持细胞生长的能力。
