Lafay Sophie, Gueux Elyett, Rayssiguier Yves, Mazur André, Rémésy Christian, Scalbert Augustin
Unité des Maladies Métaboliques and Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 St-Genes-Champanelle, France.
Int J Vitam Nutr Res. 2005 Mar;75(2):119-25. doi: 10.1024/0300-9831.75.2.119.
The effects of caffeic acid, a major phenolic compound of the diet, on oxidative stress and cholesterolemia are studied in rats submitted to oxidative stress by iron overload. Male Wistar rats were fed semi-synthetic diets containing regular (50 mg/kg diet) or high (2000 mg/kg) doses of iron with and without caffeic acid (6460 mg/kg) for 4 weeks. The high doses of iron induced an increase of lipid oxidation in the liver, as measured by thiobarbituric acid-reactive substances (TBARS), and an increase of cholesterolemia. Caffeic acid fully prevented the pro-oxidant effects of high iron doses (p < 0.001). It also reduced lipid peroxidation in rats fed the low iron dose (p < 0.05). Caffeic acid also increased vitamin E levels in plasma (2.74 micromol/L to 4.09 micromol/L for normal diet; p < 0.001; 2.78 micromol/L to 4.94 micromol/L for iron supplemented diet p < 0.001). Iron-induced hypercholesterolemia was inhibited by caffeic acid (1.07 g/L to 0.82 g/L; p < 0.001). These results demonstrate the antioxidative capacity of caffeic acid, a highly bioavailable polyphenol, in an in vivo model of oxidative stress.
饮食中的主要酚类化合物咖啡酸对铁过载所致氧化应激大鼠的氧化应激和胆固醇血症的影响进行了研究。雄性Wistar大鼠喂食含常规剂量（50毫克/千克饮食）或高剂量（2000毫克/千克）铁且添加或不添加咖啡酸（6460毫克/千克）的半合成饮食，持续4周。高剂量铁可使肝脏中的脂质氧化增加（通过硫代巴比妥酸反应性物质（TBARS）测定），并使胆固醇血症增加。咖啡酸完全预防了高剂量铁的促氧化作用（p<0.001）。它还降低了喂食低剂量铁大鼠的脂质过氧化（p<0.05）。咖啡酸还使血浆中的维生素E水平升高（正常饮食组从2.74微摩尔/升升至4.09微摩尔/升；p<0.001；铁补充饮食组从2.78微摩尔/升升至4.94微摩尔/升；p<0.001）。铁诱导的高胆固醇血症被咖啡酸抑制（从1.07克/升降至0.82克/升；p<0.001）。这些结果证明了高生物利用度多酚咖啡酸在氧化应激体内模型中的抗氧化能力。
