García-Carrasco Belén, Fernandez-Dacosta Raquel, Dávalos Alberto, Ordovás José M, Rodriguez-Casado Arantxa
IMDEA Food Institute, C/Carretera de Cantoblanco 8, 28049 Madrid, Spain.
Nutrition and Genomics Laboratory, JM-USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111-1524, USA.
Med Sci (Basel). 2015 Jun 11;3(2):38-54. doi: 10.3390/medsci3020038.
Adipose tissue dysfunction constitutes a primary defect in obesity and might link this disease to severe chronic health problems. We aimed to evaluate the antioxidant activity of three extracts from (dandelion) as well as their effects on mature 3T3-L1 adipocytes concerning intracellular lipid accumulation and cytotoxicity, this would give indications regarding therapeutic interest of dandelion as potential anti-obesity candidate. Antioxidant activities of extracts from dandelion roots and leaves were evaluated using 1,1-diphenyl-2-picrylhyorazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) methods at the concentration range used in cellular assays (300-600 µg/mL). The influence of the extracts on mature 3T3-L1 adipocyte viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Lipid content was determined by Oil-red-O staining. The extracts showed effective antioxidant activity correlating with total flavonoid and polyphenol contents. However, the functionality level was weakly associated with the antioxidant activity. Further, our data demonstrated that mature 3T3-L1 adipocytes reduced in size and number when incubated with the extracts, which suggests a significant increase in lipolysis activity. Particularly, leaf extract and crude powdered root of dandelion reduced triglyceride accumulation in mature 3T3-L1 adipocytes to a greater extent that the extract from the root. Our study shows anti-lipogenic effects of dandelion extracts on adipocytes as well as radical scavenging and reducing activity. Importantly, along with previous results indicating that cell populations cultivated in the presence of the dandelion extracts decrease in 3T3-L1 adipogenesis capacity, these results suggests that these extracts might represent a treatment option for obesity-related diseases by affecting different processes during the adipocyte life cycle.
脂肪组织功能障碍是肥胖的主要缺陷，可能将这种疾病与严重的慢性健康问题联系起来。我们旨在评估蒲公英三种提取物的抗氧化活性，以及它们对成熟3T3-L1脂肪细胞内脂质积累和细胞毒性的影响，这将为蒲公英作为潜在抗肥胖候选药物的治疗价值提供线索。使用1,1-二苯基-2-苦基肼基（DPPH）和铁还原抗氧化能力（FRAP）方法，在细胞试验中使用的浓度范围（300-600μg/mL）内评估蒲公英根和叶提取物的抗氧化活性。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验确定提取物对成熟3T3-L1脂肪细胞活力的影响。通过油红O染色测定脂质含量。提取物显示出与总黄酮和多酚含量相关的有效抗氧化活性。然而，功能水平与抗氧化活性的相关性较弱。此外，我们的数据表明，与提取物一起孵育时，成熟3T3-L1脂肪细胞的大小和数量减少，这表明脂解活性显著增加。特别是，蒲公英叶提取物和粗粉状根比根提取物在更大程度上降低了成熟3T3-L1脂肪细胞中的甘油三酯积累。我们的研究表明蒲公英提取物对脂肪细胞具有抗脂肪生成作用以及自由基清除和还原活性。重要的是，连同先前的结果表明在蒲公英提取物存在下培养的细胞群体的3T3-L1脂肪生成能力下降，这些结果表明这些提取物可能通过影响脂肪细胞生命周期中的不同过程而成为肥胖相关疾病的一种治疗选择。
