Pechkova Eugenia, Roth Stephan V, Burghammer Manfred, Fontani David, Riekel Christian, Nicolini Claudio
Fondazione ElBA, via delle Testuggini snc, I-00134 Rome, Italy.
J Synchrotron Radiat. 2005 Nov;12(Pt 6):713-6. doi: 10.1107/S0909049505011684. Epub 2005 Oct 18.
Microbeam grazing-incidence small-angle X-ray scattering (microGISAXS) has been used and the technique has been improved in order to investigate protein nucleation and crystal growth, assisted by a protein nanotemplate. The aim is to understand the protein nanotemplate method in detail, as this method has been proved capable of accelerating and increasing crystal size and quality as well as inducing crystallization of proteins that are not crystallizable by classical methods. The nanotemplate experimental setup was used for drops containing growing lysozyme crystals at three different stages of growth.
微束掠入射小角X射线散射（microGISAXS）已被用于研究蛋白质成核和晶体生长，并且该技术已经得到改进，这一过程由蛋白质纳米模板辅助。目的是详细了解蛋白质纳米模板方法，因为该方法已被证明能够加速并增加晶体尺寸和质量，还能诱导经典方法无法结晶的蛋白质结晶。纳米模板实验装置用于含有处于三个不同生长阶段的溶菌酶晶体的液滴。
