Pechkova Eugenia, Nicolini Claudio
Fondazione EL.B.A., via delle Testuggini snc, 00187 Rome, Italy.
J Cell Biochem. 2006 Feb 15;97(3):553-60. doi: 10.1002/jcb.20538.
The early steps of growth and nucleation of the lysozyme microcrystals by classical and nanotemplate-based hanging vapor diffusion methods are studied using microGISAXS at the European Synchrotron Radiation Facility (ESRF) in Grenoble, France. Out-of-plane cuts in the Yoneda regions of the 2D scattering profiles point to the detection of ultrasmall lysozyme crystals by microGISAXS quite before than by light microscopy. Furthermore lysozyme crystal formation occurs quite earlier with the nanotemplate than with the classical method. Our data are compatible with two distinct modes of crystal nucleation and growth for P450sc and lysozyme.
利用位于法国格勒诺布尔的欧洲同步辐射装置（ESRF）的微GISAXS技术，研究了通过经典的和基于纳米模板的悬滴气相扩散法进行溶菌酶微晶生长和成核的早期步骤。二维散射图谱的米氏区域中的面外切割表明，微GISAXS比光学显微镜更早检测到超小溶菌酶晶体。此外，与经典方法相比，纳米模板使溶菌酶晶体形成得更早。我们的数据与P450sc和溶菌酶两种不同的晶体成核和生长模式相符。
