Pechkova Eugenia, Sivozhelezov Victor, Tropiano Giuseppe, Fiordoro Stefano, Nicolini Claudio
Fondazione Elba, Via delle Testuggini, 00100 Roma, Italy.
Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):803-8. doi: 10.1107/S0907444905006578. Epub 2005 May 26.
The present report is dedicated to a systematic comparison of crystal structures produced by the nanobiofilm template method and by the classical hanging-drop vapour-diffusion method. Crystals grown by the innovative nanostructured template method appear indeed radiation-resistant even in the presence of a third-generation highly focused beam at the European Synchrotron Radiation Facility. The implications of this finding for protein crystallography are discussed here in terms of water redistribution and of the detailed atomic resolution comparative studies of the two crystal structures with or without nanobiofilm template, as emerging also from circular-dichroism and thermal denaturation studies.
本报告致力于对纳米生物膜模板法和经典悬滴气相扩散法所产生的晶体结构进行系统比较。通过创新的纳米结构模板法生长的晶体，即使在欧洲同步辐射装置使用第三代高聚焦光束的情况下，似乎也具有抗辐射能力。本文从水的重新分布以及对有无纳米生物膜模板的两种晶体结构进行详细的原子分辨率比较研究等方面，讨论了这一发现对蛋白质晶体学的影响，这些比较研究也来自圆二色性和热变性研究。
