Matthan Nirupa R, Welty Francine K, Barrett P Hugh R, Harausz Carrie, Dolnikowski Gregory G, Parks John S, Eckel Robert H, Schaefer Ernst J, Lichtenstein Alice H
Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington Street, Boston Mass 02111, USA.
Arterioscler Thromb Vasc Biol. 2004 Jun;24(6):1092-7. doi: 10.1161/01.ATV.0000128410.23161.be. Epub 2004 Apr 15.
To determine mechanisms contributing to decreased high-density lipoprotein cholesterol (HDL-C) and increased low-density lipoprotein cholesterol (LDL-C) concentrations associated with hydrogenated fat intake, kinetic studies of apoA-I, apoB-100, and apoB-48 were conducted using stable isotopes.
Eight postmenopausal hypercholesterolemic women were provided in random order with 3 diets for 5-week periods. Two-thirds of the fat was soybean oil (unsaturated fat), stick margarine (hydrogenated fat), or butter (saturated fat). Total and LDL-C levels were highest after the saturated diet (P<0.05; saturated versus unsaturated) whereas HDL-C levels were lowest after the hydrogenated diet (P<0.05; hydrogenated versus saturated). Plasma apoA-I levels and pool size (PS) were lower, whereas apoA-I fractional catabolic rate (FCR) was higher after the hydrogenated relative to the saturated diet (P<0.05). LDL apoB-100 levels and PS were significantly higher, whereas LDL apoB-100 FCR was lower with the saturated and hydrogenated relative to the unsaturated diet. There was no significant difference among diets in apoA-I or B-100 production rates or apoB-48 kinetic parameters. HDL-C concentrations were negatively associated with apoA-I FCR (r=-0.56, P=0.03) and LDL-C concentrations were negatively correlated with LDL apoB-100 FCR (r=-0.48, P=0.05).
The mechanism for the adverse lipoprotein profile observed with hydrogenated fat intake is determined in part by increased apoA-I and decreased LDL apoB-100 catabolism.
为确定氢化脂肪摄入导致高密度脂蛋白胆固醇（HDL-C）降低和低密度脂蛋白胆固醇（LDL-C）浓度升高的机制，采用稳定同位素对载脂蛋白A-I（apoA-I）、载脂蛋白B-100（apoB-100）和载脂蛋白B-48（apoB-48）进行了动力学研究。
8名绝经后高胆固醇血症女性被随机安排依次接受3种饮食，为期5周。三分之二的脂肪分别为大豆油（不饱和脂肪）、人造奶油（氢化脂肪）或黄油（饱和脂肪）。饱和脂肪饮食后总胆固醇和LDL-C水平最高（P<0.05；饱和脂肪与不饱和脂肪相比），而氢化脂肪饮食后HDL-C水平最低（P<0.05；氢化脂肪与饱和脂肪相比）。与饱和脂肪饮食相比，氢化脂肪饮食后血浆apoA-I水平和池大小（PS）较低，而apoA-I分数分解代谢率（FCR）较高（P<0.05）。与不饱和脂肪饮食相比，饱和脂肪和氢化脂肪饮食时LDL apoB-100水平和PS显著更高，而LDL apoB-100 FCR更低。不同饮食之间apoA-I或B-100生成率或apoB-48动力学参数无显著差异。HDL-C浓度与apoA-I FCR呈负相关（r=-0.56，P=0.03），LDL-C浓度与LDL apoB-100 FCR呈负相关（r=-0.48，P=0.05）。
氢化脂肪摄入时观察到的不良脂蛋白谱的机制部分是由apoA-I分解代谢增加和LDL apoB-100分解代谢减少所决定的。
