免疫细胞因子在自由活动大鼠中诱导的心血管反应。

Cardiovascular responses induced in free-moving rats by immune cytokines.

作者信息

Morimoto K, Morimoto A, Nakamori T, Tan N, Minagawa T, Murakami N

机构信息

Department of Biomechanics and Physiology, Faculty of Liberal Arts, Yamaguchi University, Japan.

出版信息

J Physiol. 1992 Mar;448:307-20. doi: 10.1113/jphysiol.1992.sp019043.

DOI:10.1113/jphysiol.1992.sp019043

PMID:1593469

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1176201/

Abstract

We investigated the effect of intraperitoneal (I.P.) injections of the immune cytokines, interleukin-1 beta (IL-1 beta) and tumour necrosis factor (TNF) on cardiovascular responses in free-moving rats, using a biotelemetry system. 2. The I.P. injection of a small dose of IL-1 beta (1 microgram/kg) induced a monophasic increase in the heart rate, and that of a large dose (10 micrograms/kg) induced biphasic increases in the blood pressure and heart rate. However, the I.P. injection of any of several doses of TNF (1, 10 and 50 micrograms/kg) had no effect on cardiovascular responses in rats. 3. Pre-treatment with I.P. injection of indomethacin (10 mg/kg), an inhibitor of cyclo-oxygenase, significantly suppressed the cardiovascular responses and the increase in the plasma noradrenaline (NA) concentration induced by I.P. injection of IL-1 beta. 4. Microinjection of IL-1 beta (1 and 10 ng) into the preoptic and anterior hypothalamic (PO-AH) region induced dose-dependent increases in the blood pressure and heart rate in rats. These responses were also suppressed by pretreatment with I.P. indomethacin (10 mg/kg). In addition, microinjection of prostaglandin E2 (20 and 100 ng) into the PO-AH region increased blood pressure and heart rate, but that of prostaglandin D2 (100 ng) had no effect. 5. The present results suggest that IL-1 beta stimulates the release of prostaglandins, presumably E series, near regions of the hypothalamus, which act on the hypothalamus to induce activation of the sympathetic nervous system. Subsequently, the blood pressure, heart rate and the plasma level of NA increase.

摘要

我们使用生物遥测系统，研究了腹腔注射免疫细胞因子白细胞介素-1β（IL-1β）和肿瘤坏死因子（TNF）对自由活动大鼠心血管反应的影响。2. 腹腔注射小剂量IL-1β（1微克/千克）可引起心率单相增加，大剂量（10微克/千克）则可引起血压和心率双相增加。然而，腹腔注射几种剂量的TNF（1、10和50微克/千克）对大鼠的心血管反应均无影响。3. 腹腔注射环氧化酶抑制剂吲哚美辛（10毫克/千克）进行预处理，可显著抑制腹腔注射IL-1β所诱导的心血管反应以及血浆去甲肾上腺素（NA）浓度的升高。4. 向视前区和下丘脑前部（PO-AH）区域微量注射IL-1β（1和10纳克）可引起大鼠血压和心率的剂量依赖性增加。这些反应也可被腹腔注射吲哚美辛（10毫克/千克）预处理所抑制。此外，向PO-AH区域微量注射前列腺素E2（20和100纳克）可升高血压和心率，但注射前列腺素D2（100纳克）则无影响。5. 目前的结果表明，IL-1β刺激下丘脑附近前列腺素（可能是E系列）的释放，这些前列腺素作用于下丘脑以诱导交感神经系统激活。随后，血压、心率和NA的血浆水平升高。