Kessopoulou E, Tomlinson M J, Barratt C L, Bolton A E, Cooke I D
University Department of Obstetrics and Gynaecology, Jessop Hospital for Women, Sheffield, UK.
J Reprod Fertil. 1992 Mar;94(2):463-70. doi: 10.1530/jrf.0.0940463.
Peroxidative damage induced by reactive oxygen species (ROS) has been proposed as one of the major causes of defective sperm function. In previous studies of the production of ROS in semen, the contribution of contaminating leucocytes was not assessed. We determined the levels of ROS in 60 semen samples from men attending our infertility clinic and demonstrated by performing extraction experiments with antibody-coated magnetic beads that, within this unselected population of patients, leucocytes were the major source of ROS in the low-density Percoll fraction. Of the sperm motion parameters examined using computerized semen analysis, beat-cross frequency was the only one significantly affected by the ROS in semen.
活性氧（ROS）诱导的过氧化损伤被认为是精子功能缺陷的主要原因之一。在以往关于精液中ROS产生的研究中，未评估污染白细胞的作用。我们测定了60例前来我院不孕不育门诊就诊男性的精液样本中的ROS水平，并通过用抗体包被的磁珠进行提取实验证明，在这群未经选择的患者中，白细胞是低密度Percoll组分中ROS的主要来源。在使用计算机化精液分析检测的精子运动参数中，拍击交叉频率是唯一受精液中ROS显著影响的参数。
